Establishment of Fingerprint of Classic Prescription Qianghuo Shengshi Decoction and Predictive Analysis of Functional Substances
Objective:Based on the method of network pharmacology and the establishment of the fingerprint of the classic prescription Qianghuo Shengshi Decoction(QSD),the quality marker(Q-marker)of QSD was analyzed and predicted.Method:The fingerprint of QSD was established by HPLC.The common peaks were assigned and identified,and the active components were screened based on measurability and traceability.The target and pathway were screened by network pharmacology,and the"component-target-pathway"interaction network diagram of QSD was constructed to predict the Q-marker of QSD.Results:A total of 20 common peaks were calibrated.Ten chromatographic peaks were identified by comparing with the reference substances,including prim-O-glucosylcimifugin,senkyunolide Ⅰ,osthole,nodakenin,glycyrrhizic acid ammonium salt,ligustilide,ferulic acid,isoimperatorin,5-O-methylvisammioside and columbianadin,and the similarities were all greater than 0.862.The"component-target-pathway"network of six active components was constructed and analyzed by network pharmacology,the enriched pathways included proteoglycans in cancer,pathways in cancer,chemical carcinogenesis-receptor activation,endocrine resistance and so on.According to the connectivity between components,targets and pathways,it was preliminarily predicted that columbianadin,prim-O-glucosylcimifugin,glycyrrhizic acid ammonium salt,isoimperatorin,osthole and 5-O-methylvisammioside may achieve the functions of expelling wind,eliminating dampness and relieving pain by regulating these signal pathways.Conclusion:By establishing the QSD fingerprint to analyze and predict the Q-marker in QSD based on network pharmacology methods,the quality control standards of QSD are further clarified,which is beneficial for improving its quality control level,and also provides a reference for the in-depth study of the mechanism of action of QSD in the later stage.
万方数据
维普
