Biological Characteristics of the Pathogen Causing Septoria Leaf Spot of Scutellaria baicalensis and Screening of Fungicides
Objective:To study the biological characteristics of Corynespora cassiicola,the pathogen causing Septoria leaf spot of Scutellaria baicalensis and screen the agents for efficient control of this disease in the cultivation and production of S.baicalensis.Methods:The influences of carbon and nitrogen sources,culture medium,temperature,pH,and lighting conditions on C.cassiicola and the toxicities of five commercial fungicides against C.cassiicola were examined by the mycelial growth rate method.Results:The optimal growth conditions for C.cassiicola were the carrot medium supplemented with soluble starch and sucrose as carbon sources and peptone as the nitrogen source at pH value of 6 and temperature of 30℃.The lethal treatments for the mycelia and spores were 51℃for 10 min and 54℃for 10 min,respectively.The toxicity of the five fungicides against C.cassiicola followed the descending order of 98%carbendazim,98%fludioxonil,98%prochloraz,98%pyraclostrobin,and 98%azoxystrobin with the median effect concentrations(EC50)of 0.07 mg·L-1,0.10,0.18,3.06,and 632.39 mg·L-1,respectively.Conclusions:C.cassiicola can survive under a wide range of conditions.It could grow with a variety of carbon and nitrogen sources,culture media,and pH conditions and demonstrated rapid growth in neutral to weak acid environment and at high temperatures.Carbendazim and fludioxonil showed strong inhibitory effects on C.cassiicola and can be used for controlling Septoria leaf spot of S.baicalensis in the field.
Scutellaria baicalensis GeorgiSeptoria leaf spotCorynespora cassiicolabiological characteristicsscreening of fungicides
万方数据
维普
