Establishment of UPLC Fingerprint of Standard Vladimiriae Radix Decoction and Quantitative Analysis of Multiple Indicator Components
Objective:To establish a quality control method for standard Vladimiriae Radix decoction.Methods:The ultra-high performance liquid chromatography(UPLC)column Waters CORTECS T3 was used.Gradient elution was carried out using acetonitrile-0.1%phosphoric acid solution as the mobile phase.The flow rate of the mobile phase was 0.35 mL·min-1,and the column temperature was 40℃.The detection wavelength was 325 nm at 0-21 min and 225 nm at 21-37 min.The chromatographic injection volume was 1 μL.14 batches of standard Vladimiriae Radix decoction fingerprints were established by UPLC to determine the common peaks in the fingerprints.The common peaks were confirmed using reference substances.Similarity calculation and chemometrics methods were used to analyze the standard decoction prepared by Vladimiriae Radix from different regions,and four effective components,namely costunolide,dehydrocarbactone,chlorogenic acid,and 4,5-di-O-caffeoylquinic acid were quantitatively analyzed.Results:A total of 14 common peaks were identified in the fingerprints of standard Vladimiriae Radix decoction,and four components were identified:costunolide,dehydrocarbactone,chlorogenic acid,and 4,5-di-O-caffeoylquinic acid.Similarity evaluation,cluster analysis(HCA),and principal component analysis(PCA)all divided the 14 batches of standard Vladimiriae Radix decoction into two categories,and orthogonal partial least squares-discriminant analysis(OPLS-DA)screened a total of seven differential markers from the 14 common peaks.The content determination results showed that the standard decoction prepared by Vladimiriae Radix from Aba,Sichuan,had the highest total content of costunolide and dehydrocarbactone,while the total content of chlorogenic acid and 4,5-di-O-caffeoyl quinic acid was the lowest.The overall difference between Vladimiriae Radix from the other three regions was not significant.Conclusion:This method has a short analysis time,high accuracy,and good specificity,and it can provide a reference for the formulation of quality standards for standard Vladimiriae Radix decoction and its related preparations.
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