Determination of Four Preservatives in Fresh Bamboo Juice by UPLC-PDA Method
To establish a ultrahigh performance liquid chromatography-diode array detector(UPLC-PDA)method to detect the contents of four commonly used preservatives in commercially available fresh bamboo juice,including benzoic acid,sorbic acid,methylparaben,and ethylparaben.Methods:The ACQUITY UPLC HSS T3 column(100 mm×2.1 mm,1.8 µm)was used with a gradient elution of acetonitrile as mobile phase A and 0.02 mol·L-1 ammonium acetate as mobile phase B at a flow rate of 0.3 mL·min-1.The 223 nm(benzoic acid)and 254 nm(sorbic acid,methylparaben and ethylparaben)were used as detection wavelengths,the injection volume was 1 µL,and the column temperature was 30℃.Results:The four preservatives were all completely separated at their determination wavelengths,and the average recoveries of the four preservatives were 97.9%,104.0%,99.2%and 103.0%,respectively;the RSDs(n=6)of the peak areas of benzoic acid and ethylparaben in the repeatability test were both<1%;the mass concentrations of the four preservatives showed a good linear relationship with the peak area(r all>0.999 45);and the samples had a good stability.The RSD of the peak area of benzoic acid within 48 h was 1.47%,and the RSD of the peak area of ethylparaben within 48 h was 0.2%.The preservatives in 13 batches of fresh bamboo juice were detected,benzoic acid was detected in 9 batches of fresh bamboo juice(mass fraction of 0.07%-0.29%),sorbic acid was detected in 2 batches of fresh bamboo juice(mass fraction of 0.21%-0.24%),benzoic acid(mass fraction of 0.21%-0.22%)and ethylparaben(mass fraction of 0.025%-0.027%)were detected in 2 batches of fresh bamboo juice,all of which met the requirements in Section of Mixture of the General Rule 0181 in the Chinese Pharmacopoeia(2020 version)Volume Ⅳ.Conclusion:A method for determining the contents of four commonly used preservatives in fresh bamboo juice is established based on UPLC-PDA,and the method has good specificity,reproducibility and accuracy,which provides a reference for the quality control of fresh bamboo juice,a product which is not used on a large scale.
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