Antioxidant Activity of Codonopsis Radix in vitro from Different Origins
Objective:The study is aimed to determine the content of polysaccharide,flavonoids,saponin and the antioxidant activity in vitro in 58 batches of Codonopsis Radix samples,and to comprehensively evaluate the relationship between the origin,the content of active ingredients and the antioxidant activity.Methods:A UV-visible spectrophotometry method was established to determine the content of 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH),and 2,2-diazo-bis(3-ethyl-benzothiazole-6-sulfonic acid)diamiammonium salt(ABTS),the relationship between origin,active ingredient content and antioxidant activity was analyzed by cluster analysis,bivariate correlation analysis,partial least squares-regression analysis and gray correlation analysis.Results:The mass concentration of total flavonoids in 58 batches of Codonopsis Radix samples was 0.418-1.396 mg·mL-1;the mass concentration of total polysaccharide was 4.954-15.822 mg·mL-1;and the mass concentration of total saponins was 12.111-52.052 mg·mL-1.Cluster analysis and partial least squares-regression analysis showed that the 58 batches of Codonopsis Radix could be divided into two categories.The first category was Codonopsis Radix from Chongqing,Hubei,Sichuan,Inner Mongolia,and Shanxi,and the second was Codonopsis Radix from Weiyuan County,Lintao County,Zhangxian County,Longnanwen County,and Danchang County of Gansu Province,and the antioxidant activity was affected by the origin.The results of bivariate correlation analysis showed that flavonoids may be a significant factor affecting antioxidant activity.The grey correlation analysis showed that the content of flavonoids was closely related to the antioxidant activity of Codonopsis Radix,and the results were consistent with the bivariate correlation analysis.Conclusion:All 58 batches of Codonopsis Radix have certain antioxidant activity,and the antioxidant activity is affected by the origin and the content of active ingredients.
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