Physiological Response of Saposhnikovia divaricate to Cold Stress in Spring
Objective:To investigate the physiological response of Saposhnikovia divaricate to cold stress in spring and determine the best harvest time of S.divaricate according to the content of secondary metabolites.Methods:The fresh roots of S.divaricate were harvested at the end of autumn and in spring,respectively,and the relationships between the reactive oxygen species(ROS)content,antioxidant enzyme activities,key enzyme activity of secondary metabolites,secondary metabolite content,total polysaccharide content,and acid polysaccharide content were investigated.Results:Affected by the low temperatures in spring,the content of ROS and malondialdehyde(MDA),the activities of superoxide dismutase(SOD),catalase(CAT),and peroxidase(POD),the activities of key enzymes of secondary metabolites including phenylalanine ammonia-lyase(PAL),acetyl CoA carboxylase(ACC),and chalcone synthase(CHS),and the content of 1,3-disphosphoglycerate(1,3-DPG),a product of the metabolic pathway in the fresh roots of S.divaricate were significantly increased compared with those in autumn.Compared with that in autumn,the content of prim-O-glucosylcimifugin,5-O-methylvisamminol,and cimifugin in spring was increased by 17.7%,36.7%,and 11.1%,respectively,while the content of total polysaccharide and acid polysaccharide in autumn was higher than that in spring.Conclusion:The antioxidant enzymes and secondary metabolites of S.divaricate synergistically maintain the balance of ROS under cold stress in spring,and spring harvest can increase the content of chromone.
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