首页|指纹图谱、多成分定量与模式识别相结合的预知子(三叶木通)配方颗粒质量评价

指纹图谱、多成分定量与模式识别相结合的预知子(三叶木通)配方颗粒质量评价

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
目的:建立指纹图谱、多成分定量分析和化学模式识别相结合的预知子(三叶木通)配方颗粒质量评价方法.方法:制备预知子(三叶木通)标准汤剂和配方颗粒,建立高效液相色谱法指纹图谱,对其中 8 个主要成分含量进行测定,并计算转移率和出膏率.进行相似度评价、聚类分析(HCA)、主成分分析(PCA)、偏最小二乘法-判别分析(PLS-DA),评价配方颗粒和标准汤剂化学成分的一致性.结果:预知子(三叶木通)标准汤剂中新绿原酸、绿原酸、隐绿原酸、木通苯乙醇苷B、异绿原酸B、异绿原酸A、异绿原酸C、木通皂苷D质量分数分别为1.94～10.33、2.18～13.77、1.77～9.15、2.31～19.34、1.77～6.48、1.16～5.55、2.55～9.90、0.92～8.93 mg·g-1,转移率分别为 36.25%～56.02%、24.64%～40.23%、35.88%～58.41%、29.45%～50.69%、16.55%～29.54%、26.25%～46.59%、25.33%～41.66%、11.03%～17.35%,出膏率为 13.59%～24.49%.预知子(三叶木通)配方颗粒指标成分含量、转移率、出膏率与标准汤剂接近,指纹图谱相似度、HCA、PCA、PLS-DA评价表明,配方颗粒和标准汤剂化学成分一致性较好.结论:预知子(三叶木通)配方颗粒与标准汤剂化学成分一致性较好,结果合理、可靠,可为其工艺优化及质量评价提供参考.
Quality Evaluation of Akebiae Frucus[Akebia trifoliate(Thunb.)Koidz.]Dispensing Granules by Combination of Fingerprint,Multi-Component Quantitative Analysis,and Chemical Pattern Recognition
Objective:To establish a quality evaluation method for Akebiae Frucus[A.trifoliate(Thunb.)Koidz.]dispensing granules by combining fingerprinting,multi-component quantitative analysis,and chemical pattern recognition.Methods:Standard Akebiae Frucus[A.trifoliate(Thunb.)Koidz.]decoctions and dispensing granules were prepared.High-performance liquid chromatography(HPLC)fingerprinting was established,and the contents of eight components were determined.The transfer rate and extraction rate were calculated,and similarity evaluation,cluster analysis(HCA),principal component analysis(PCA),and partial least squares discriminant(PLS-DA)were performed to evaluate the consistency of chemical components between dispensing granules and standard decoctions.Results:The mass fractions of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,calceolarioside B,isochlorogenic acid B,isochlorogenic acid A,isochlorogenic acid C,and akebia saponin D in standard Akebiae Frucus[A.trifoliate(Thunb.)Koidz.]decoctions were 1.94-10.33,2.18-13.77,1.77-9.15,2.31-19.34,1.77-6.48,1.16-5.55,2.55-9.90,and 0.92-8.93 mg·g-1,respectively.The transfer rates of these components were 36.25%-56.02%,24.64%-40.23%,35.88%-58.41%,29.45%-50.69%,16.55%-29.54%,26.25%-46.59%,25.33%-41.66%,and 11.03%-17.35%.The extraction rate was 13.59%-24.49%.The component content,transfer rate,and extraction rate of Akebiae Frucus[A.trifoliate(Thunb.)Koidz.]dispensing granules were close to those of standard decoctions.The results of similarity evaluation,HCA,PCA,and PLS-DA indicated good consistency of chemical components between dispensing granules and standard decoctions.Conclusion:There was good consistency between dispensing granules and standard decoctions of Akebiae Frucus[A.trifoliate(Thunb.)Koidz.].The results were reasonable and reliable,which can provide a reference for process optimization and quality evaluation.

Akebia trifoliate(Thunb.)Koidz.standard decoctionsdispensing granulesfingerprintmulti-component quantitative analysischemical pattern recognition

曹桂云、宁波、田汝芳、张风超、庄雪松、张京华、林永强、董晓弟、李金鑫、孟兆青

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山东宏济堂制药集团股份有限公司,山东 济南 250103

山东省食品药品检验研究院,山东 济南 250101

三叶木通 标准汤剂 配方颗粒 指纹图谱 多成分定量分析 化学模式识别

山东省重点研发订划重大科技创新工程项目泉城"5150"引才倍增计划项目(2021年)

2021CXGC010511

2024

10.13313/j.issn.1673-4890.20240124001

中国现代中药
中国中药协会,中国医药集团总公司,中国药材公司

中国现代中药

CSTPCD
影响因子：0.65
ISSN：1673-4890
年,卷(期)：2024.26(6)
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