Objective:To establish a method for the content determination of 13 components in Tongkang Tablets,and comprehensively evaluate the quality of Tongkang Tablets by chemometrics and grey relational analysis(GRA).Method:HPLC-quantitative analysis of multi-components by single marker(QAMS)method was used,with acetonitrile-methanol(9:1)-0.5%phosphoric acid aqueous solution as mobile phase for gradient elution;the detection wavelengths were 300 nm(for narirutin and hesperidin),254 nm(for prim-O-glucosylcimifugin,cimifugin,sec-O-glucosylhamaudol,calycosin 7-O-β-D-glucopyranoside,ononin and 9,10-dimethoxy-pterocarpane-3-O-β-D-glucoside),203 nm(for astragaloside Ⅳ and diosgenin),and 220 nm(for allantoin,atractylenolide Ⅲ and atracylenolide Ⅰ),respectively.Chemometrics and GRA were used for differential analysis of the results of multi-index component content.Results:The results of methodological validation all met the requirements in the Chinese Pharmacopoeia(2020 edition).Chemometric analysis showed that hesperidin,9,10-dimethoxy-pterocarpane-3-O-β-D-glucoside,prim-O-glucosylcimifugin,atractylenolide Ⅲ and allantoin were the main potential markers affecting the quality of Tongkang Tablets.The relative correlation degree of GRA was 0.297 6-0.667 8,and the quality difference of Tongkang Tablets produced in the same period was small.Conclusion:The HPLC-QAMS method can simultaneously determine the contents of 13 components in Tongkang Tablets,which is simple and practical.Chemometrics and GRA can be used to evaluate the overall quality of Tongkang Tablets.
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