Detection of Panax ginseng Adulteration in Bushen Yinao Capsules by UPLC-MS/MS
Objective:This study aims to establish an ultra-high performance liquid chromatography-mass spectrometry(UPLC-MS/MS)method for determining Panax quinquefolium illegally added in Bushen Yinao Capsule preparation and to investigate whether enterprises use P.quinquefolium or its leftover ingredients to replace Panax ginseng,so as to provide technical support for traditional Chinese medicine(TCM)supervision.Methods:Phenomenex C18 column(100 mm×2.1 mm,2.6 μm)was used,with 10 mmoL·L-1 ammonium acetate l(A)-acetonitrile(B)as the mobile phase for gradient elution.The flow rate was 0.3 mL·min-1,and the column temperature was 40℃.Electrospray negative ion mode(ESI-)was employed for multiple reaction monitoring(MRM).Results:Pseudo-ginsenoside F11,a special component of P.quinquefolium,was detected in 29 batches of 60 Bushen Yinao Capsules,with a detection rate of 48.3%.Conclusion:There was P.ginseng C.A.Mey.adulteration in Bushen Yinao Capsules.The method was accurate and reliable and could be used as a supplementary test method for P.quinquefolium illegally added in Bushen Yinao Capsules.
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