Determination of Starch Content in Ginseng by Dual-wavelength Specrophotometry
Objective:To determine the content of amylose and amylopectin in ginseng by dual-wavelength spectrophotometry.Methods:Amylose and amylopectin in ginseng were taken as the main research objects.The content of amylose and amylopectin in ginseng from forests and gardens in different years was determined,and the distribution pattern was analyzed.The determination conditions and methods were optimized by using the dual-wavelength spectrophotometry in iodine colorimetry,and finally,the accuracy of the best method was verified by methodology.Results:According to the principle of the dual-wavelength method and the scanning photograms,the determined wavelengths and reference wavelengths of amylose were 600 nm and 471 nm,and those of amylopectin were 540 nm and 702 nm,respectively.There was a good linear relationship(r=0.999 8,r=0.999 7)between amylose and amylopectin concentrations in the ranges of 0.004-0.060 mg·mL-1 and 0.02-0.16 mg·mL-1.The average spiked recoveries were 100.03%and 101.84%,and the RSDs were 1.29%and 1.92%,respectively.Conclusion:The method is easy to operate,with good accuracy,stability,and reproducibility,and it is suitable for the determination of amylose and amylopectin content in ginseng.
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