基于UPLC特征图谱和含量测定的不同产地筋骨草质量评价
Quality Evaluation of Ajuga decumbens from Different Producing Areas Based on UPLC Characteristic Chromatogram and Content Determination
丘娴 1周湘媛 1罗佩琪 1彭娇 1谢远远 1马懿飞 1何民友 1李振雨 1陈向东 1孙冬梅1
作者信息
- 1. 广东一方制药有限公司,广东 佛山 528244;广东省中药配方颗粒企业重点实验室,广东 佛山 528244
- 折叠
摘要
目的:建立筋骨草超高效液相色谱法(UPLC)特征图谱和其中哈巴苷、乙酰哈巴苷的含量测定方法.方法:采用Waters BEH C18 色谱柱(150 mm×2.1 mm,1.7 μm),以乙腈-甲醇(4∶1)为流动相A、0.2%磷酸水溶液为流动相B,梯度洗脱,流速为 0.30 mL·min-1,柱温为 30℃,检测波长为 202 nm,进样量为 2 μL,建立筋骨草特征图谱,测定其中哈巴苷、乙酰哈巴苷的含量.结果:17 批筋骨草特征图谱有 10 个共有峰,指认了其中 4个成分分别为哈巴苷、乙酰哈巴苷、木犀草素-7-二葡萄糖苷酸、芹菜素-7-二葡萄糖苷酸;17 批筋骨草中哈巴苷质量分数为 0.33%～0.95%,乙酰哈巴苷质量分数为 0.47%～1.93%.结论:建立的UPLC特征图谱和哈巴苷、乙酰哈巴苷含量测定方法可为筋骨草质量评价提供参考.
Abstract
Objective:To establish the characteristic chromatogram of Ajuga decumbens Thunb.by ultra-high performance liquid chromatography(UPLC)and method for determining the content of harpagoside and acetyl harpagoside.Methods:Waters BEH C18(150 mm×2.1 mm,1.7 μm)column was used.The mobile phase A was acetonitrile-methanol(4:1),and the mobile phase B was 0.2%phosphoric acid aqueous solution for gradient elution.The flow rate was 0.30 mL·min-1,and the column temperature was 30℃.The detection wavelength was 202 nm,and the injection volume was 2 μL.The characteristic chromatogram of A.decumbens was established to determine the content of harpagoside and acetyl harpagoside.Results:A total of 10 common fingerprint peaks were identified in the characteristic chromatograms of 17 batches of A.decumbens,among which four components were identified as harpagoside,acetyl harpagoside,luteonin-7-diglucosidic acid,and apigenin-7-diglucosidic acid.The mass fraction of harpagoside in the 17 batches of A.decumbens.was in the range of 0.33%-0.95%,while that of acetyl harpagoside was 0.47%-1.93%.Conclusion:The established UPLC characteristic chromatogram and the method for determining the content of harpagoside and acetyl harpagoside can provide a basis for the quality evaluation of A.decumbens.
关键词
筋骨草/超高效液相色谱法/特征图谱/含量测定Key words
Ajuga decumbens Thunb./UPLC/characteristic chromatogram/content determination引用本文复制引用
基金项目
工业和信息化部2022年产业技术基础公共服务平台项目(2022-230-221)
佛山市南海区重点领域科技攻关专项(南科[2023]20号-18)
出版年
2024
国家科技期刊平台
NSTL
万方数据