Effective Components and Mechanism of Yinjia Tablets in Treating Pelvic Inflammatory Disease
Objective:To study the effective components and mechanism of Yinjia Tablets in treating pelvic inflammatory disease.Methods:The chemical components of Yinjia Tablets were identified by ultra-performance liquid chromatography-quadrupole-Exactive Orbitrap-mass spectrometry(UPLC-Q-Exactive Orbitrap MS)combined with comparison with literature data and reference substances.The common targets shared by the chemical components and pelvic inflammatory disease were imported into String database to construct the protein-protein interaction(PPI)network.Gene ontology(GO)enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed with DAVID.Cytoscape 3.9.1 was used to establish the"medicinal material-component-target-pathway"network.Molecular docking was performed in Maestro 11.9.Results:A total of 58 chemical components were identified in Yinjia Tablets,including flavonoids,organic acids,terpenoids,and alkaloids.Five core targets were obtained by the PPI network analysis,including steroid receptor coactivator(SRC),heat shock protein 90 alpha family class A member 1(HSP90AA1),phosphoinositide-3-kinase regulatory subunit 1(PIK3R1),signal transducers and activators of transcription 3(STAT3),and mitogen-activated protein kinase 3(MAPK3).The KEGG analysis results showed that Yinjia Tablets mainly exerted the therapeutic effect via phosphatidylinositol 3-kinase-protein kinase B(PI3K-Akt),MAPK and other pathways.Molecular docking results showed stable binding of luteoloside,kaempferol,luteolin,and diosmetin with five core targets including SRC,HSP90AA1,and PIK3R1.Conclusion:Yinjia Tablets may play a role in the treatment of pelvic inflammatory disease by regulating the PI3K-Akt and MAPK pathways via the active components such as luteoloside,kaempferol,luteolin,diosmetin,and apigenin.
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