Optimization of the Extraction Process of Total Flavonoids from Ficus erecta by Response Surface Methodology and Its Antioxidant Activity
Objective:To optimize the extraction process and determine the antioxidant activity of total flavonoids from Ficus erecta(Thunb.)var.beecheyana(Hook.et Arn.)King.Methods:Four factors,namely ethanol concentration,material-liquid ratio,extraction time,and number of extractions,were selected to obtain the optimal conditions through response surface methodology and mathematical modeling,thus improving the extraction rate of total flavonoids from F.erecta.The in vitro antioxidant activity was investigated,measuring 1,1-Diphenyl-2-picrylhydrazyl radical(DPPH),2,2-azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS),and hydroxyl radical scavenging capacities.Results:The optimal extraction process for total flavonoids from F.erecta was identified with 60%ethanol,1:23 material-liquid ratio,and 18-min extraction time,under which the extraction rate was 16.25 mg·g-1.The scavenging rates of total flavonoids from F.erecta for DPPH,ABTS,and hydroxyl radicals reached 92.84%,89.34%,and 69.73%,respectively.Conclusion:The optimized extraction process was operable,stable,and reliable,exhibiting good antioxidant activity.
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