HPLC Specific Chromatogram of Standard Decoction of Genkwa Flos Processed by Vinegar
Objective:To establish the high performance liquid chromatography(HPLC)specific chromatogram of standard decoction of Genkwa Flos processed by vinegar,comprehensively characterize its composition characteristics,and distinguish it from the standard decoction of Genkwa Flos.Methods:The Kromasil Cl8 chromatographic column(250 mm×4.6 mm,5 μm)was used.Methyl alcohol was used as mobile phase A,and an aqueous solution containing 0.05%phosphoric acid was used as mobile phase B for gradient elution.The flow rate was 1.0 mL·min-1,and the column temperature was 35 °C.The injection volume was 20 µL,and the detection wavelength was 238 nm.The obtained specific chromatograms of 21 batches of standard decoction of Genkwa Flos and Genkwa Flos processed by vinegar were matched by"Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System"(2004A version)in terms of peaks,and the number of common peaks was determined,respectively.The contrast specific chromatograms were generated.By comparison,the difference in the specific chromatograms of the standard decoction of Genkwa Flos and Genkwa Flos processed by vinegar were defined,and the common peaks were identified.The changes in the peak area of the identified characteristic peaks were compared.Results:By comparing two contrast specific chromatograms of the standard decoction of Genkwa Flos and Genkwa Flos processed by vinegar,it was found that one characteristic peak with a retention time of 27.0 min was the distinguishing point,suggesting that this component may be a new olefinic compound from the volatile oil of Genkwa Flos processed by vinegar.The seven characteristic peaks were identified as apigenin-7-O-glucuronide,genkwanin-5-O-rubiin,luteolin,bassin,apigenin,hydroxygenkwanin,and genkwanin,and there was no obvious change in their content before and after processing.Conclusion:The established method is simple,feasible,and targeted,which can distinguish the standard decoction of Genkwa Flos and Genkwa Flos processed by vinegar.It can lay a foundation for the quality control of standard decoction and formula granules of Genkwa Flos processed by vinegar while strengthening the quality control of samples from the source.
Genkwa FlosGenkwa Flos processed by vinegarstandard decoctionspecific chromatogramquality control
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