Quality Control and Evaluation Method of Changtongshu Granules
Objective:To establish the quality control and evaluation method of Changtongshu Granules.Methods:Thin-layer chromatography was used to qualitatively identify Polygoni Multiflori Radix,Angelicae Sinensis Radix,and Mori Frucuts in Changtongshu Granules.The contents of albiflorin,paeoniflorin,2,3,5,4ʹ-tetrahydroxystilbene-2-O-β-D-glucoside,and benzoic acid were simultaneously determined by high-performance liquid chromatography(HPLC).The contents of the above four components were taken as comprehensive indexes,and a grey correlation analysis method was established to evaluate the quality of Changtongshu Granules.Results:The spots of the corresponding medicinal materials were clearly visible under the thin-layer chromatogram,with good separation and no interference from the negative samples.Albiflorin,paeoniflorin,2,3,5,4ʹ-tetrahydroxystilbene-2-O-β-D-glucopyranoside,and benzoic acid had a good relationship in their respective linear ranges.The average recovery was 100.19%-100.80%,and the relative standard deviation(RSD)was 0.48%-0.90%.The relative correlation degree obtained by the optimal and worst evaluation unit sequence was between 0.334 and 0.675,indicating that the quality of each sample was different.Among them,S3,S2,and S5 rank the top three,and the quality of the sample was relatively good.Conclusion:The established qualitative research method of thin-layer chromatography has good durability and specificity.The comprehensive evaluation model method of simultaneous determination of four index components and grey correlation is objective,accurate,and reliable,which can provide new ideas for the quality control and evaluation method of Changtongshu Granules.
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