Quality Analysis of Liandou Qingmai Mixture by TLC and Multi-component Quantitation
Objective:To establish a comprehensive quality control method for Liandou Qingmai Mixture based on multi-component determination and thin layer chromatography(TLC).Methods:Forsythiae Fructus,Coptidis Rhizoma,and Paeoniae Radix Rubra in Liandou Qingmai Mixture were identified by TLC.The contents of forsythoside and berberine in the mixture were determined by high-performance liquid chromatography(HPLC)using an XBridge C18 column,with acetonitrile-0.6%phosphoric acid aqueous solution as mobile phase at a flow rate of 0.7 mL·min-1,column temperature of 25℃,and detection wavelength of 277 nm.Results:The TLC showed clear spots for Forsythiae Fructus,Coptidis Rhizoma,and Paeoniae Radix Rubra with appropriate Rf values and no negative interference.The content determination indicated that forsythoside and berberine had a good linear relationship in the range of 14.84-148.36 μg·mL-1 and 5.72-57.22 μg·mL-1(r=0.999 3),respectively,with average recoveries of 99.07%(RSD 2.75%)and 99.86%(RSD 3.60%),respectively.The concentration of forsythoside in the three batches was 409.63,402.29,and 378.86 μg·mL-1,and that of berberine was 205.61,201.73,and 163.02 μg·mL-1 separately.Conclusion:The established TLC and HPLC methods for multi-components were scientific and effective and could be used as the quality control standard for Liandou Qingmai Mixture.
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