Objective:To simultaneously determine the content of aristolochic acid Ⅰ and aristolochic acid Ⅱ in Shuangxin Bidouyan Granules,and provide a reference for their quality control.Methods:Ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was used to simultaneously determine the content of aristolochic acid Ⅰ and aristolochic acid Ⅱ in Shuangxin Bidouyan Granules.A Waters ACQUITY UPLC HSS T3 column(100 mm×2.1 mm,1.8 μm)was used with a gradient elution of acetonitrile(A)and 0.1%formic acid in water(containing 1 mmol·L-1 ammonium acetate,B)as the mobile phase.An electrospray ionization(ESI)source in positive ion multiple reaction monitoring mode was employed.The content of aristolochic acid Ⅰ and aristolochic acid Ⅱ in the samples were calculated using the standard curve method.Results:Among 12 batches of Shuangxin Bidouyan Granules,aristolochic acidⅠ was not detected in 9 batches(detection limit 0.13 pg),while it was detected in 3 batches with a mass fraction ranging from 14.62 to 25.82 ng·g-1.Aristolochic acid Ⅱ was not detected in any of the samples(detection limit 0.44 pg).Conclusion:The established UPLC-MS/MS method for the simultaneous determination of aristolochic acid Ⅰ and aristolochic acid Ⅱ in Shuangxin Bidouyan Granules is highly specific,sensitive,and reproducible.This method provides a reference for the quality control of aristolochic acid Ⅰ and aristolochic acid Ⅱ in Shuangxin Bidouyan Granules.
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