Content Determination of Aristolochic Acid Ⅰ in Three Chuanxiong Chatiao Products by UPLC-MS/MS
Objective:An analytical method was established to simultaneously determine and compare the content of aristolochic acid Ⅰ in three dosage forms of Chuanxiong Chatiao products,Chuanxiong Chatiao Pills,Chuanxiong Chatiao Pills(concentrated pills)and Chuanxiong Chatiao Granules,by ultra-performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).Methods:The samples were separated on ACQUITY UPLC BEH C18(100 mm×2.1 mm,1.7 μm)column and gradient elution was performed with acetonitrile-0.1%formic acid solution as a mobile phase.The flow rate was 0.3 mL·min-1,and triple quadrupole tandem mass spectroscopy was used as the detector.The samples were detected under multiple reaction monitoring(MRM)mode with electrospray ionization(ESI)in positive ion mode,and the mass to charge ratio(m/z)359.0→298.1 and 359.0→296.1 ion pairs were selected for monitoring.The contents of aristolochic acid Ⅰin three Chuanxiong Chatiao products from 48 batches were determined and the precision,stability and repeatability were verified.Results:The contents of aristolochic acid Ⅰ had a good linear relationship with the peak area when the mass concentration was 2.96-295.82 ng·mL-1,and the r value of the three products were all greater than 0.999.The average recoveries were 93.36%,85.08%,90.13%,and RSDs were10.04%,6.04%,11.85%,respectively.The limit of quantitation(LOQ)was 0.040 6-0.157 7 μg·g-1.Conclusion:This method has good accuracy,precision,stability and linearity,and is suitable for the detection and analysis of aristolochic acid Ⅰ in Chuanxiong Chatiao preparation.The results suggest that for the processing and preparation of traditional Chinese medicine containing toxic components such as aristolochic acid,it is more appropriate to use non original powder as the medicine.
Chuanxiong Chatiao serial productsdosage formUPLC-MS/MSaristolochic acid Ⅰcontent determination
万方数据
维普
