Content Determination of Aristolochic Acid Ⅰ and Aristolochic Acid Ⅱ in Aristolochia moupinensis Franch.by UPLC-MS/MS
Objective:To establish an ultrahigh performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method for the content determination of aristolochic acid Ⅰ(AA-Ⅰ)and AA-Ⅱ in Aristolochia moupinensis Franch.Methods:The chromatographic column was Agilent Poroshell SB-C18 column(100 mm×2.1 mm,2.7 μm);the mobile phase was acetonitrile-0.1%formic acid aqueous solution(containing 5 mmol·L-1 ammonium formate)with gradient elution;the flow rate was 0.3 mL·min-1;the column temperature was 35℃;the ion source was electrospray ion source;the scanning mode was positive ion scanning with multiple reaction monitoring(MRM)mode.Results:AA-Ⅰshowed a good linear relationship with the peak area at a mass concentration of 1.036-207.310 ng·ml-1(r=0.999 5).AA-Ⅱshowed a good linear relationship with the peak area at a mass concentration of 1.1-110.0 ng·ml-1(r=0.999 1).The limit of quantification(LOQ)and limit of detection(LOD)of AA-Ⅰ were 0.328 7 and 0.098 6 pg,respectively,and the LOQ and LOD of AA-Ⅱ were 0.650 6 and 0.195 2 pg,respectively.The RSDs of precision,stability and repeatability tests of AA-Ⅰwere all less than 5.0%,and those of AA-Ⅱ were all less than 5.0%.The average spiked recovery of AA-Ⅰ was 92.50%with RSD of 4.3%.The average spiked recovery of AA-Ⅱ was 104.97%with RSD of 3.7%.Among the 15 batches of A.moupinensis Franch,no AA-Ⅰ was detected in 1 batch,and no AA-Ⅱ was detected in 8 batches.The content of AA-Ⅰ in the remaining batches was 5.94×10-4-2.86 mg·g-1,and that of AA-Ⅱ was 0.09-0.50 mg·g-1.Conclusion:The established method is specific,rapid,sensitive,and can be used for the content determination of AA-Ⅰ and AA-Ⅱ in A.moupinensis Franch..
万方数据
维普
