Compositional Differences of Lilii Bulbus Based on Thin Layer Identification and Fingerprint
Objective:To establish thin-layer chromatography(TLC)and fingerprint methods for the identification of three original medicinal materials(Lilium lancifolium Thunb.,L.brownii F.E.Brown var.viridulum Baker,and L.pumilum DC.)of Lilii Bulbus and their corresponding honey-processed products and to study the differences in their chemical components.Methods:The study employed an Ultimate XB C18 chromatographic column(250 mm×4.6 mm,5 μm)with a mobile phase of acetonitrile(A)and 0.1%phosphoric acid aqueous solution(B)in a gradient elution.High-performance liquid chromatography(HPLC)fingerprint profiles were established for 21 batches of different original medicinal materials of Lilii Bulbus.Similarity evaluation,cluster analysis,principal component analysis,and orthogonal partial least squares-discriminant analysis(OPLS-DA)were performed to assess the similarities and differences among different Lilium species.For TLC,silica gel G plates were used with ethyl acetate-methanol-formic acid-water(18:2:1.5:1)and ether-n-butanol-glacial acetic acid(3:9:6)as the developing agents to establish differentiation methods for different original Lilium medicinal materials and honey-processed products.This comprehensive approach aimed to display the chemical composition characteristics of different original Lilium species and honey-processed products.Results:The fingerprint profiles showed significant differences among the different original Lilium medicinal materials.Samples of L.lancifolium Thunb.,L.brownii F.E.Brown var.viridulum Baker,and L.pumilum DC.had 8,12,and 8 peaks,respectively.Common components included homopantothenic acid,regaloside H,and regaloside A.Regaloside I existed in L.brownii F.E.Brown var.viridulum Baker,and L.pumilum DC.,while 2-acetate regaloside A existed in L.lancifolium Thunb..The similarity evaluation results(all less than 0.50)indicated considerable differences in their components.Statistical analysis revealed that the different original Lilium species were clustered into distinct groups.Peaks 12(2-acetyl regaloside A),13,14,11(regaloside I),and 3(homopantothenic acid)were identified as differential components among L.lancifolium Thunb.,L.brownii F.E.Brown var.viridulum Baker,and L.pumilum DC.TLC results demonstrated significant differences in chromatographic spots among different original Lilium species,which corroborated the fingerprint profile results.Additionally,TLC of honey-processed products showed spots for sucrose,fructose,and glucose,while Lilium medicinal materials only had sucrose spots,indicating the differences arising from the honey-processing.Conclusion:The established fingerprint and TLC methods can effectively evaluate the quality of Lilium medicinal materials.These methods provide a quick,accurate,and intuitive way to reflect the differences in components among different original Lilium species and honey-processed products,offering reference standards for quality control and differentiation of various Lilium medicinal materials and honey-processed products.
Lilii Bulbusprocessing with honeydifferent originsTLCfingerprintdistinguish and identify
万方数据
维普
