Analysis of Differential Components of Polygonum milletii and the Confused Product Bergenia scopulosa Based on PLS-DA
Objective:To identify the differential components and establish a methodology for content determination of Polygonum milletii and its homonym herb Bergenia scopulosa.Methods:Partial least squares discriminant analysis(PLS-DA)combined with ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometry(UPLC-Q-TOF-MS)was used to analyze the differential components of P.milletii and B.scopulosa and to establish a methodology for their qualitative and quantitative determination.Results:The PLS-DA results showed that 296 differential components of P.milletii and B.scopulosa were identified using the t-test(P<0.05)and VIP value(VIP>1.0)as criteria.The top-ranked differential components were characterized and analyzed,resulting in the identification of eight differential components that conformed to fragmentation patterns and literature.Among these,the content differences in three components,i.e.,chlorogenic acid,gallic acid,and bergenin,were the most significant.Conclusion:This method can effectively analyze the differences between P.milletii and B.scopulosa and provide a reference for the quality evaluation and qualitative and quantitative analysis of P.milletii and B.scopulosa.
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