Determination of Contentof Ten Active Componentsin Four Plant Species Producing Zangyinchen by HPLC
Objective:To establish a method based on high performance liquid chromatography(HPLC)for simultaneous determination of 10 active components(swertiamarin,mangiferin,gentiopicroside,sweroside,isoorientin,isovitexin,swertisin,swertianolin,swertiaperennin,and oleanolic acid)in Zangyinchen.Methods:The chromatographic separation was achieved bygradient elutionthrougha Welch Ultimate XB-C18 column with acetonitrile-0.1%phosphoric acid solution as the mobile phaseat the flow rate of 1.0 mL·min-1.The detection wavelength was set at 240 nm for swertiamarin,mangiferin,gentiopicroside,and sweroside,270 nm for isoorientin,isovitexin,and swertisin,250 nm for swertianolin andswertiaperennin,and 205 nm for oleanolicacid.The volume of sample injection was 10 μL.Results:The ten active components were well separated.Swertiamarin,mangiferin,gentiopicroside,sweroside,isoorientin,isovitexin,swertisin,swertianolin,swertiaperennin,andoleanolic acidshowed good linearity with the peak areaswithin the ranges of 126.7-16 040.0 ng(r=0.999 8),6.3-801.6 ng(r=0.999 6),6.4-814.4 ng(r=0.999 7),15.9-2 016.0 ng(r=0.999 9),7.1-900.0 ng(r=0.999 5),5.5-699.2 ng(r=0.999 7),5.4-681.6 ng(r=0.999 8),5.4-685.6 ng(r=0.999 6),19.3-2 438.0 ng(r=0.999 7),and 13.6-1 716.0 ng(r=0.999 8),respectively.The established method demonstrated good precision and repeatability,with the relative standard deviations(RSDs)≤0.95%and≤1.86%,respectively.The test solutions were stable in 24 h,withthe RSDs no more than 1.85%.The average spiked recovery and corresponding RSDs were 99.56%(0.58%),100.37%(1.23%),98.63%(0.59%),99.11%(1.02%),98.64%(0.57%),102.26%(0.88%),100.37%(0.59%),97.05%(1.36%),96.01%(0.45%),and 101.55%(0.49%),respectively.The content of swertiamarin,mangiferin,gentiopicroside,sweroside,isoorientin,isovitexin,swertisin,swertianolin,swertiaperennin,andoleanolic acid in 18 batches of Zangyinchen samples was 0.111%-6.592%,0.195%-1.959%,0.046%-4.139%,0.058%-0.662%,0.090%-1.421%,0.082%-0.283%,0.061%-0.384%,0.022%-1.589%,0.106%-1.858%,and 0.160%-0.657%,respectively.Conclusion:An HPLC method was established for the simultaneous determination of ten components in Zangyinchen,being simple,quick,and accurate.The findings lay a foundation for the quality control of Zangyinchen and promote the comprehensive applicationof the plants.
high performance liquid chromatography(HPLC)Zangyinchenactive componentcontent determination
万方数据
维普
