中国现代中药2024，Vol.26Issue(9) ：1535-1543.DOI:10.13313/j.issn.1673-4890.20240202001

防风多糖SP-3抗肿瘤活性及其机制研究

Antitumor Activity and Mechanism of Saposhnikovia divaricata Polysaccharide SP-3

王凯赫 秦铭 王石峰 刘湉 母磊鑫 柯彩依 徐广 马群
中国现代中药2024，Vol.26Issue(9) ：1535-1543.DOI:10.13313/j.issn.1673-4890.20240202001
✕
  • 维普
  • 万方数据

防风多糖SP-3抗肿瘤活性及其机制研究

Antitumor Activity and Mechanism of Saposhnikovia divaricata Polysaccharide SP-3

王凯赫 1秦铭 1王石峰 1刘湉 1母磊鑫 1柯彩依 1徐广 1马群1
扫码查看

作者信息

  • 1. 北京中医药大学 中药学院,北京 102488
  • 折叠

摘要

目的:研究防风多糖SP-3 的抗肿瘤活性及作用机制.方法:通过噻唑蓝(MTT)法、流式细胞术检测防风多糖SP-3 对癌细胞增殖、细胞周期及凋亡的影响,采用秀丽隐杆线虫(以下简称线虫)模型研究防风多糖SP-3 抗肿瘤活性机制.结果:防风多糖SP-3 可以显著抑制胃癌AGS细胞、肝癌HepG2 细胞的增殖,促进胃癌AGS细胞的凋亡并影响其细胞周期,且呈剂量依赖性;防风多糖SP-3 对大鼠肉瘤(Ras)基因突变的线虫多阴门表型具有显著的抑制作用.结论:防风多糖SP-3 具有良好的抗肿瘤效果,其作用机制可能与Ras/丝裂原活化蛋白激酶(MAPK)通路有关.

Abstract

Objective:To study the antitumor activity and mechanism of Saposhnikovia divaricata polysaccharide SP-3.Methods:The effects of SP-3 on cancer cell proliferation,cell cycle,and apoptosis were detected using the MTT assay and flow cytometry.The antitumor activity mechanism of SP-3 was studied using the Caenorhabditis elegans model.Results:SP-3 significantly inhibited the proliferation of gastric cancer AGS cells and hepatoma HepG2 cells,promoted apoptosis in gastric cancer AGS cells,and affected their cell cycle in a dose-dependent manner.Additionally,SP-3 exhibited a notable inhibitory effect on the multivulva phenotype in Ras gene mutant C.elegans.Conclusion:SP-3 has a potent antitumor effect,and its mechanism may be related to the Ras/mitogen-activated protein kinase(MAPK)pathway.

关键词

防风多糖SP-3/胃癌AGS细胞/秀丽隐杆线虫/大鼠肉瘤/丝裂原活化蛋白激酶

Key words

Saposhnikovia divaricata polysaccharide SP-3/gastric cancer AGS cells/Caenorhabditis elegans/Ras/MAPK

引用本文复制引用

出版年

2024
中国现代中药
中国中药协会,中国医药集团总公司,中国药材公司

中国现代中药

CSTPCD
影响因子：0.65
ISSN：1673-4890
段落导航相关论文