Investigating Anti-Alzheimer's Disease Activity of Kadsura Pepper Stem Polysaccharide Based on Model Organism of Caenorhabditis Elegans
Objective:To explore the anti-Alzheimer's disease(AD)activity of fractionated polysaccharides from Kadsura Pepper Stem(KPSP).Methods:KPSP(KPSP1-KPSP3)was prepared with three ethanol precipitation concentrations(40%,60%,and 80%)using graded ethanol precipitation.The sugar content of KPSP was determined using the phenol-sulfuric acid method.Caenorhabditis elegans CL4176(hereafter referred to as nematodes)was used as a model organism to observe the effects of KPSP on nematode lifespan,paralysis,stress capacity,and contents of reactive oxygen species(ROS),superoxide anion radical and β-amyloid protein(Aβ)in nematodes.Results:The sugar mass fractions in KPSP1,KPSP2 and KPSP3 were(57.45±0.27)%,(65.32±1.26)%,and(70.26±3.53)%,respectively.The results showed that KPSP at the optimal concentration prolonged the nematode lifespan by 38.35%,42.02%,and 39.13%,respectively.To a certain extent,it slowed the paralysis rate of the nematodes and reduced the contents of ROS,superoxide anion radical,and Aβ in the nematodes.Conclusion:KPSP exhibits good antioxidant activity and anti-Alzheimer's disease activity.
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