摘要
目的:研究喷施不同叶面肥对柔毛淫羊藿开花结实及黄酮醇苷类成分含量的影响.方法:以柔毛淫羊藿 2 年生苗为供试材料,采用 0.1%磷酸二氢钾(T1)、0.06%硼酸(T2)、0.1%磷酸二氢钾+0.03%硼酸(T3)、5%氨基酸水溶肥(T4)及清水对照(CK)5 个处理,分别于抽薹前、花蕾期、盛花期喷施,分析各处理对植株光合作用、开花结实和叶中黄酮醇苷成分含量的影响.结果:与CK比较,T1 处理能够显著增加花枝数、种子产量和总黄酮醇苷含量;T2 处理能够增加花枝数、花枝长度、种子产量、种子千粒质量及叶片中朝藿定A、朝藿定B含量;T3 处理能够增加花枝数和种子产量,但不利于黄酮醇苷类成分的积累;T4 处理能够增加花枝数、种子产量和总黄酮醇苷含量.结论:合理施用叶面肥可促进柔毛淫羊藿开花结实、提高黄酮醇苷类成分含量,其中硼酸促进结实效果最佳,磷酸二氢钾和氨基酸水溶肥对柔毛淫羊藿生长和黄酮醇苷类成分积累有积极作用.研究结果可为柔毛淫羊藿种子生产和药材定向培育施肥方案的制定提供参考.
Abstract
Objective:To study the effects of different foliar fertilizer sprays on flowering and fruiting and flavonol glycosides content in Epimedium pubescens.Methods:Two-year-old seedlings of E.pubescens were used as test materials.Five treatments were applied:0.1%potassium dihydrogen phosphate(T1),0.06%boric acid(T2),0.1%potassium dihydrogen phosphate+0.03%boric acid(T3),5%amino acid water-soluble fertilizer(T4)and water(CK).These were sprayed before budding,at the bud stage,and in full bloom.The effects of each treatment on photosynthesis,flowering,and fruiting,and the content of flavonol glycosides in leaves were analyzed.Results:Compared with CK,T1 treatment significantly increased the number of flowering branches,seed yield,and total flavonol glycosides;T2 treatment increased the number and length of flowering branches,seed yield,thousand-seed weight,and the content of epimedin A and epimedin B in leaves;T3 treatment increased the number of flowering branches and seed yield,but was not conducive to the accumulation of flavonol glycosides;T4 treatment increased the number of flowering branches,seed yield and total flavonol glycoside.Conclusion:Rational application of foliar fertilizer can promote flowering and fruiting and increase the content of flavonol glycosides.Among the treatments,boric acid is most effective in promoting fruiting,while potassium dihydrogen phosphate and amino acid water-soluble fertilizers have a positive effect on growth and flavonol glycosides accumulation.The results of this study can provide a reference for the seed production of E.pubescenum and the formulation of fertilization programs for the targeted cultivation of medicinal materials.
维普
万方数据