Chloroplast Whole Genome Analysis and DNA Barcode Construction of Patriniae Herba and Its Related Species
Objective:To develop specific DNA barcodes based on the chloroplast genome sequence analysis of the original plants of Patrinia medicinal materials to identify Patrniae Herba accurately and its related species.Methods:The chloroplast genomes of three species of the Patrinia plants,namely P.villosa,P.scabiosaefolia,and P.heterophylla,were sequenced using Illumina high-throughput sequencing technology.Bioinformatics software was employed for genome assembly,annotation,feature analysis,sequence comparison,and phylogenetic analysis.Specific DNA barcodes were constructed based on the highly variable regions of the chloroplast genome and were validated.Results:The chloroplast genomes of the three Patrinia plants exhibited a quadripartite structure,with total lengths of 159 585 bp,158 919 bp,and 158 919 bp,respectively.They encoded 130,132,and 132 genes,including 8 rRNA genes and 37 tRNA genes.The number of protein-coding genes was 85,87,and 87,respectively.The ccsA,ndhG,rpl23,and ndhF sequences could be used as specific DNA barcodes,successfully amplifying 16 samples.P.scabiosaefolia,P.villosa,and P.monandra clustered together in the same branch,while P.heterophylla and P.scabra clustered in another branch.The ccsA,ndhG,and ndhF sequences further distinguished P.heterophylla from P.scabra.Conclusion:The sequences of ccsA,ndhG,rpl23,and ndhF can be used as supplementary specific DNA barcodes to distinguish Patriniae Herba and its related species.
万方数据
维普
