UPLC-Q-TOF-MS Identifies Components of Oligosaccharides with Antioxidant Activity in Polygonati Rhizoma before and after Processing
Objective:To explore the components,changes,and antioxidant activity of oligosaccharides in Polygonati Rhizoma before and after processing with wine.Methods:The composition of the oligosaccharides derivatized with 1-pheny-3-methyl-5-pyrazolone(PMP)was analyzed by ultra-performance liquid chromatography-quadrupole time-of-flight high-resolution mass spectrometry.The content of oligosaccharides was determined with the phenol-sulfuric acid method.The antioxidant activity of PMP-derivatized oligosaccharides in vitro was evaluated based on the 1,1-diphenyl-2-picrylhydrazine(DPPH)and diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)(ABTS+)free radical scavenging activities,and that in vivo was determined in Caenorhabditis elegans.Results:Three oligosaccharides(sucrose,1-kestose and nystose)were detected in both raw and processed Polygonati Rhizoma.The content of sucrose,1-kestose,and nystose decreased after processing,and raffinose,melibiose,1F-fructofuranosylnystose,and kestohexaose were not detected after processing.The content of glucose,fructose,and galactose increased after processing.The content of oligosaccharides in raw and processed Polygonati Rhizoma was 78.17%and 70.80%,respectively.The median effective concentrations(EC50)of oligosaccharides in raw and processed Polygonati Rhizoma were 2.12 mg·mL-1 and 0.41 mg·mL-1 against DPPH free radicals and 2.90 mg·mL-1 and 0.95 mg·mL-1 against ABTS+free radicals,respectively.The oligosaccharides in both raw and processed Polygonati Rhizoma alleviated the heat stress and oxidative stress damage in C.elegans.Conclusion:The composition of oligosaccharides in Polygonati Rhizoma changed after processing.Polygonati Rhizoma oligosaccharides demonstrated antioxidant activity both in vitro and in vivo,and the activity was enhanced after Polygonati Rhizoma was processed with wine.The above results provide a basis for explaining the effect changes of Polygonati Rhizoma before and after processing and have reference significance for the development and utilization of Polygonati Rhizoma.
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