Biological Effects of Different Medicinal Properties of Sojae Semen Praeparatum with Medicinal and Food Attributes Based on the Differences in Gut Microorganisms in Normal Rats
Objective:To investigate the regulatory effects of Sojae Semen Praeparatum(SSP)prepared with different medicinal excipients through fermentation processes on the gut microbiota of normal rats.Methods:SD rats were randomly divided into a control group,a raw soybean group,a fermentation group without excipients,and fermentation groups with excipients(Group Ⅰ:Artemisiae Annuae Herba+Mori Folium;Group Ⅱ:Ephedrae Herba+Perillae Folium;group Ⅲ:Qingwen Jiedu Decoction+Artemisiae Annuae Herba).After 6 weeks of administration of different extracts of raw soybeans or SSP,cecum contents were collected,and 16S rRNA sequencing was used to observe changes in the gut microbiota of each group.The PICRUSt tool was employed to analyze the functional differences in the microbiota among the groups.Results:Compared with raw soybeans,consuming SSP increased the diversity of the gut microbiota.Compared with the fermentation group without excipients,consuming SSP fermented with different medicinal excipients reduced the species composition of the microbiota,decreased the relative abundance of Lactobacillus,and increased the relative abundance of Ruminococcaceae_UCG_005.The comparison between the groups with added excipients showed that the group with warm excipients had lower abundances of Lactobacillus and Ruminococcaceae_UCG_005 than the other two groups.The groups with cold and cool excipients showed similar changes at the phylum and genus levels.KEGG enrichment results indicated that the relative abundance of gut microbiota in functional categories such as"circulatory system"was significantly reduced in the cold excipient fermentation group,while in the warm excipient fermentation group,the relative abundance in functional categories such as"chemical structure transition diagram"and"transporter"was significantly reduced.The cool excipient fermentation group showed significant reductions in the relative abundance of gut microbiota in functional categories such as"chemical structure transition diagram"and"circulatory system",closely related to sugar biosynthesis and metabolism,transport,and catabolism.Conclusion:There is an objective biological basis for the medicinal and edible uses of SSP.In dietary health,SSP can moderately increase the diversity of gut microorganisms and play a role in disease prevention.In pharmacotherapy,SSP can help reduce the incidence of immune-related diseases.SSP prepared with different medicinal excipients has different advantages in the prevention and treatment of inflammatory,neurological,and metabolic diseases.
Sojae Semen Praeparatummedicinal propertiescecal contentsmedicinal and edible uses
万方数据
维普
