Regulatory Effects of Callianthemum taipaicum W.T.Wang on Inflammatory Factors and Intestinal Flora in Young Rats with Pneumonia
Objective:To study the effects of Callianthemum taipaicum W.T.Wang on the intestinal flora and short-chain fatty acid(SCFA)metabolism in young rats with pneumonia and to explore its potential mechanisms of action.Methods:Changes in intestinal flora in the feces of SD young rats were detected using 16S rRNA high-throughput sequencing.The content of SCFAs,including acetic acid,propionic acid,butyric acid,isobutyric acid,and isovaleric acid in feces,was determined by gas chromatography-mass spectrometry.Results:C.taipaicum significantly increased the body weight of young rats(P<0.01),reduced the levels of inflammatory factors TNF-α and IL-8 in lung tissue(P<0.05),decreased the lung index(P<0.01),and improved lung injury.It also significantly alleviated disturbances in the intestinal flora of the young rats,restored the diversity of intestinal flora,increased the relative abundance of beneficial bacteria,and elevated the content of acetic acid and propionic acid in feces(P<0.05).At the phylum level,C.taipaicum primarily led to a decrease in the relative abundance of Firmicutes and an increase in the relative abundance of Bacteroidetes.At the class level,the relative abundance of Bacteroidia increased,while the relative abundance of Clostridia tended to be similar to that of the control group.At the order level,the relative abundance of Bacteroidales increased,while that of Clostridiales decreased.At the family level,the relative abundance of Prevotellaceae significantly increased,while that of Lachnospiraceae and Ruminococcaceae significantly decreased.At the genus level,there was an increasing trend in the relative abundance of Prevotella_9,Lactobacillus,Prevotellaceae_Ga6A1_group,Prevotella_1,Spirillum NK4A136 group,and Prevotella_UCG-001,while the relative abundance of Bacteroides,Faecalibacterium,and[Eubacterium]_rectale_group decreased.Conclusion:C.taipaicum can improve the levels of inflammatory factors and the diversity of intestinal flora in young rats with pneumonia by regulating the composition of intestinal flora and the content of SCFAs.
万方数据
维普
