Mechanism of Wenyang Tongluo Prescription in Regulating Synovial Fibroblasts in Rheumatoid Arthritis Based on MiR-26b-3p
Objective:To investigate the regulatory effect of Wenyang Tongluo Prescription on the proliferation,migration,and invasion of synovial fibroblasts in rheumatoid arthritis based on miR-26b-3p.Methods:SD rats were randomly divided into a Wenyang Tongluo Prescription group and a blank serum group.The Wenyang Tongluo Prescription group was given Wenyang Tongluo Prescription at 4.05 g·kg-1·d-1,twice daily,with a 12-hour interval,for 3 consecutive days.Blood was collected from the abdominal aorta 1 hour after the last administration,followed by serum isolation.The Cell Counting Kit-8(CCK-8)assay was used to determine the optimal concentration and intervention time of Wenyang Tongluo Prescription-containing serum.Human rheumatoid arthritis synovial fibroblasts(MH7A)were transfected with inhibitor NC,miR-26b-3p inhibitor,mimic NC,and miR-26b-3p mimic,respectively,and treated with Wenyang Tongluo Prescription-containing serum.Human fibroblast-like synovial cells(HFLS)were set as the blank control group.Real-time quantitative PCR(qRT-PCR)was used to detect the expression of miR-26b-3p in MH7A cells.The proliferation of cells in each group was detected by the CCK-8 assay.The migration and invasion of cells in each group were detected by the Transwell assay.Results:The expression of miR-26b-3p was upregulated by serum containing Wenyang Tongluo Prescription at different concentrations(P<0.05),and the expression of miR-26b-3p increased in a concentration-dependent manner.After the intervention with the drug-containing serum of Wenyang Tongluo Prescription,the proliferation,migration,and invasion abilities of MH7A cells were weakened(P<0.05).After miR-26b-3p overexpression,the proliferation,migration,and invasion of MH7A cells were weakened(P<0.05).After interfering with the expression of miR-26b-3p,the proliferation,migration,and invasion of MH7A cells were enhanced(P<0.05).On the basis of interfering with the expression of miR-26b-3p,the proliferation,migration,and invasion of MH7A cells were weakened after intervention with the drug-containing serum of Wenyang Tongluo Prescription(P<0.05).Conclusion:Wenyang Tongluo Prescription can inhibit the proliferation,migration,and invasion of synovial fibroblasts in rheumatoid arthritis by upregulating the expression of miR-26b-3p.
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维普
