Fingerprint and Content Determination of Siphonostegia chinensis Benth.Standard Decoction
Objective:To establish a high-performance liquid chromatography(HPLC)fingerprint and multi-component quantitative analysis method for Siphonostegia chinensis Benth.standard decoction.Methods:The HPLC method was used to establish the fingerprint of S.chinensis Benth.standard decoction,and the similarity was evaluated using the Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System(2012 version).Cluster analysis(CA),principal component analysis(PCA),and orthogonal partial least squares-discriminant analysis(OPLS-DA)were performed to screen marker compounds influencing quality differences based on a variable importance projection(VIP)value>1.The content of luteolin-7-O-β-D-glucuronide,verbascoside,and luteolin was determined by HPLC.Results:The fingerprint of S.chinensis Benth.standard decoction showed 11 common peaks,with a similarity greater than 0.90 among all samples.Peak 1 was identified as caffeic acid,peak 3 as luteolin-7-O-β-D-glucuronide,peak 4 as verbascoside,peak 6 as apigenin-7-O-β-D-glucuronide,peak 7 as isoacteoside,peak 10 as luteolin,and peak 11 as apigenin.Cluster analysis divided the 18 batches of samples into four categories:S16-S18,S4 and S12,S9,S11,and S14,and the remaining batches clustered into one group.Principal component analysis showed that the cumulative variance contribution rate of the three principal components was 89.019%.OPLS-DA indicated that peaks 11,9,7,5,4,and 10 were key components affecting the quality of S.chinensis Benth.standard decoction.The content of verbascoside,luteolin-7-O-β-D-glucuronide,and luteolin in the 18 batches of S.chinensis Benth.standard decoction ranged from 0.38%to 2.17%,0.13%to 0.73%,and 0.06%to 0.21%,respectively.Conclusion:The established fingerprint and quantitative analysis methods are simple to operate and have good repeatability and reproducibility.They can be used to evaluate the quality differences of the standard decoction of S.chinensis Benth.and have certain significance for its quality control and holistic evaluation.
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