Rapid and Non-destructive Determination of Wuwei Qingzhuo Preparation Based on Hyperspectral Technology
Objective:To determine quantitatively the content of piperine,Cinnamic aldehyde,and hydroxysafflor yellow A in Wuwei Qingzhuo Preparation of Mongolian medicine by hyperspectral technology combined with chemometrics for rapid,non-destructive,and comprehensive quality assessment of Wuwei Qingzhuo Preparation.Methods:Thirty-three batches of Wuwei Qingzhuo Preparation samples,including 11 batches of Wuwei Qingzhuo San and 22 batches of Wuwei Qingzhuo pills,were selected from the National Drug Sampling and Inspection Plan in 2023 to collect hyperspectral data of the samples.The hyperspectral quantitative calibration model was compared with the performance of 10 spectral pre-processing methods,including multiple scattering correction(MSC),baseline correction,normal variable transformation(SNV),spectrum transformation(ST),vector normalization,noise filtering,Savitzky-Golay(SG)9-1st smoothing,SG9-2nd smoothing,SG11-1st smoothing and SG11-2nd smoothing,2 variable selection methods,including competitive adaptive reweighted sampling(CARS)and Monte Carlo uninformative variable elimination(MC-UVE),as well as 2 modeling methods,including partial least squares(PLS)and least square support vector machine(LS-SVM)for the determination of piperine,Cinnamic aldehyde and hydroxysafflor yellow pigment A content.Results:The LS-SVM models of piperine and Cinnamic aldehyde constructed by the CARS method had the best predictive ability,and the relative predictive deviations(RPD)of the models were 9.2 and 6.0,respectively;the correlation coefficients of the validation sets(rpre)were 0.993 5 and 0.985 2,indicating that the validation sets of the models had a good nonlinear relationship with the measured values and that the models were ideal for prediction.The LS-SVM model established by the raw spectra of hydroxysafflor yellow pigment A was the most effective,with the RPD and rpre of 3.7 and 0.976 2,respectively.Conclusion:With hyperspectral technology combined with chemometrics,the content of piperine,Cinnamic aldehyde,and hydroxysafflor yellow pigment A in Wuwei Qingzhuo Preparation can be rapidly determined.The method was easy to operate and was useful for the quality control of Wuwei Qingzhuo Preparation.
万方数据
维普
