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鸭跖草及其伪品竹节菜的数字化鉴定分析

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目的:基于超高效液相色谱-四级杆飞行时间质谱法(UPLC-QTOF-MS)和数字"身份证",实现鸭跖草及其伪品竹节菜的数字化鉴定分析.方法:利用UPLC-QTOF-MS对鸭跖草、竹节菜进行分析.经Progenesis QI量化后,分别提取不同批次鸭跖草、竹节菜的共享离子信息,筛选得到各自的特异性离子数组,并根据离子强度输出前n个离子信息分别作为鸭跖草、竹节菜的数字"身份证".以上述数字"身份证"为基准进行样品的匹配鉴定,反馈匹配可信度.结果:基于鸭跖草和竹节菜的数字"身份证",能够高效快速地实现两者在个体水平上的数字化鉴定,且鸭跖草和竹节菜的匹配可信度均大于 68.00%,即使混合样品中含有 5%的竹节菜也能被高效、准确地鉴定识别.结论:基于UPLC-QTOF-MS和数字"身份证",能够在个体水平上实现鸭跖草及其伪品竹节菜的快速数字化鉴定分析,有利于鸭跖草的质量控制,为中药数字化质量控制建设提供了新的思路和参考.
Digital Identification and Analysis of Commelinae Herba and Its Counterfeit Commelina diffusa
Objective:To achieve digital identification and analysis of Commelinae Herba(CH)and its counterfeit Commelina diffusa(CD)using ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-QTOF-MS)and digital"identity".Methods:UPLC-QTOF-MS was utilized to analyze CH and CD.After quantification with Progenesis QI,the shared ion information from different batches of CH and CD was extracted,and their specific ion arrays were screened.The top n ion information based on ion intensity was output as the digital"identity"of CH and CD,respectively.This digital"identity"was then used as a benchmark for sample matching identification,with feedback provided on the matching credibility.Results:The digital"identity"of CH and CD enabled efficient and rapid digital identification of the two at the individual level,with matching credibility exceeding 68.00%.Even when CD made up only 5%of mixed samples,it could still be identified efficiently and accurately.Conclusion:Based on UPLC-QTOF-MS and digital"identity",rapid digital identification and analysis of CH and its counterfeit CD can be achieved at the individual level.This method facilitates rapid quality control of CH and offers new ideas and references for the digital quality control of Chinese medicine.

Commelinae Herbadigitizationdigital"identity"identification and analysisUPLC-QTOF-MS

张佳婷、王献瑞、贺方良、荆文光、郭晓晗、李明华、余坤子、杨建波、程显隆、魏锋

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中国食品药品检定研究院 中药民族药检定所,北京 102629

国家药品监督管理局 药品监管科学全国重点实验室,北京 102629

中国药科大学 中药学院,江苏 南京 211198

鸭跖草 数字化 数字"身份证" 鉴定分析 超高效液相色谱-四级杆飞行时间质谱法

2024

中国现代中药
中国中药协会,中国医药集团总公司,中国药材公司

中国现代中药

CSTPCD
影响因子:0.65
ISSN:1673-4890
年,卷(期):2024.26(12)