Quantity Transfer Law of Diandanshen Standard Decoction
Objective:This paper aims to establish ultra-performance liquid chromatography(UPLC)content determination and characteristic spectrum method for Diandanshen standard decoction and study the quantity transfer law of Diandanshen decoction pieces,standard decoction,and formula granules.Methods:15 batches of Diandanshen standard decoction were prepared.A method for determining the UPLC content of rosmarinic acid as an indicator component was established,and the content of rosmarinic acid in each batch of standard decoction was determined to calculate its transfer rate.UPLC characteristic spectrums of standard decoction were built,and cluster analysis and principal component analysis were performed.Based on the consistency of the standard decoction extract rate,indicator component content,and characteristic spectrum,three batches of Diandanshen formula granules were prepared to study the quantity transfer of the indicator component,namely rosmarinic acid in Diandanshen decoction slices,standard decoction,and formula granules,as well as the variation of characteristic peaks in the characteristic spectrum.Results:The content of rosmarinic acid in 15 batches of Diandanshen standard decoction is 0.36%-0.83%,and the transfer rate is 31.72%-60.57%.The UPLC characteristic spectrum of the standard decoction has a total of five characteristic peaks,with a similarity being greater than 0.9.Cluster analysis is divided into two categories.The cumulative variance contribution rate of the two principal components is 98.005 2%.All five characteristic peaks in the decoction pieces are transferred to the standard decoction,and peaks 1 and 2 are identified as rosmarinic acid and caffeic acid,respectively.The rosmarinic acid content,transfer rate,and characteristic spectrum of the three batches of Diandanshen formula granules are close to the standard decoction prepared from the same batch of decoction pieces,and all are within the quality standard range.Conclusion:The UPLC content determination and characteristic spectrum of decoction pieces,standard decoction,and formula granules based on Diandanshen standard decoction established in this study comprehensively reflect the transfer law of the three values,and quality control can be carried out throughout the entire process of preparing Diandanshen formula granules.
Diandanshen formula Granulesstandard decoctionrosmarinic acidUPLCcharacteristic spectrumcluster analysisprincipal component analysis
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