辛伐他汀治疗子痫前期大鼠的疗效观察
Therapeutic effect of simvastatin on rats with pre-eclampsia
张晓娟 1周瑞民 1邢红霞2
作者信息
- 1. 457000,河南省濮阳市第三人民医院
- 2. 新乡医学院第一附属医院
- 折叠
摘要
目的 探讨辛伐他汀对子痫前期大鼠的治疗效果.方法 选取SD雌性孕鼠共30只,随机分为正常组、子痫前期组和辛伐他汀组(每组10只).于孕14 d,分别给子痫前期组和辛伐他汀组大鼠尾静脉注入内毒素(1.0μg/kg),建立子痫前期模型,辛伐他汀组大鼠同时给予灌胃给药辛伐他汀片[40 mg/(kg·d)]治疗;正常组大鼠尾静脉注入等量生理盐水.孕第8、15、19天测定各组大鼠24 h尿蛋白含量;孕第10、15、19天测定各组大鼠心率、血压;孕第21天用硝酸还原酶法测定各组大鼠血浆一氧化氮(NO)浓度.结果①辛伐他汀组、子痫前期组、正常组大鼠24 h尿蛋白含量(孕第19天尿蛋白含量-孕第8天尿蛋白含量)分别为(73±20)、(464±57)、(140±12)μg,3组两两比较,差异有统计学意义(P＜0.01).②3组大鼠血压(孕第19天血压-孕第10天血压)比较,差异也有统计学意义(P＜0.01).③3组大鼠心率(孕第19天心率-孕第10天心率)比较,差异无统计学意义(P＞0.05).④孕第21天,血浆NO浓度正常组为(158±31)mmol/L,子痫前期组为(49±36)mmol/L,辛伐他汀组为(162±14)mmol/L,子痫前期组分别与其他两组比较,差异均有统计学意义(P＜0.01).结论 辛伐他汀可以提高子痫前期模型大鼠体内NO水平,降低血压和尿蛋白含量,对心率没有明显影响.
Abstract
Objective To study the effect of simvastatin on the nitrogenm onoxide (NO) concentration in rats with pre-eclampsia.Methods Thirty SD rats were selected and divided into three groups: normal pregnancy group,pre-eclampsia model group and simvastatin group (10 rats in each group).We used endotoxin to set up the pre-eclampsia model rats on gestation 14th day and simvastatin to treat these rats.The 24 hour urine protein on gestation 8th,15th,19th days were determined,as well as the heart rate,blood pressure on gestation 10th,15th,19th days.Nitrate reductase method was used to determine the plastion between the simvastatin [(73±20 )μg],pre-eclampsia [(464±57) μg],and normal pregnancy [(140±12)μg]groups were significant differences in blood pressure(which is equal to the value of blood pressure of pregnancy 19th day subtracting that mong the three groups(which was equal to the value of heart rate of pregnancy 19th day subtracting that of pregnancy 10th day,8th day) among the three groups(P＜0.01),and the value of normal pregnancy group was(158±31) mmol/L,pre-eclampsia model group(49±36)mmol/L,simvastatin group ( 162±14)mmol/L.Conclusions Simvastatin can elevate the level of NO concentration and decrease the blood pressure and urineprotein,but not the heart rate in pre-eclampsia model rats,which suggests that simvastatin maybe used to treat pre-eclampsia.
关键词
先兆子痫/辛伐他汀/一氧化氮/大鼠Key words
Pre-eclampsia/Simvastatin/Nitricoxide/Rats引用本文复制引用
出版年
2009
NETL
NSTL
维普
万方数据