Yindan xinnaotong capsule regulates AKT to inhibit neointimal hyperplasia:mechanism study based on network pharmacological analysis
Objective To investigate the intervention effect of Yin Dan Xin Nao Tong Ruan Jiao Nang(YDX-NT)on neointimal hyperplasia by integrating the strategy of network pharmacology and experiment in vivo.Methods The active constituents of YDXNT and their targets were retrieved from TCMSP and Symmap databases.Disease targets of neointimal hyperplasia were obtained from OMIM,DisGeNET and GeneCards databases,and the intersection of targets for YDXNT and neointimal hyperplasia was selected.Cytoscape software was used to construct and visualize the"component-disease-target-pathway"network for intersection genes.Metascape was used for GO and KEGG enrichment analysis,CytoNCA plug-in was used to obtain core targets,and autodock software was used for molecular docking between the core targets and active ingredients.The neointimal hyper-plasia model was made by balloon injury method and YDXNT was give for intervention.Hematoxylin-eosin(HE)staining was used to analyze the changes of blood vessel morphology,and the expression of core target protein was detected by immunohistochemistry.Results The network pharmacological analysis suggested that YDXNT could act on AKT1,a key target of neointimal hyperplasia,through quercetin,luteotin,baicalein and other pharmaco-logical components.HE results showed that YDXNT could effectively inhibit neointimal hyperplasia.Immunohis-tochemical results showed that the expression of AKT and p-AKT(Thr308)protein in the model group was high-er than that in the sham operation group.The protein expression of AKT and p-AKT(Thr308)in YDXNT group was down-regulated compared with that in model group.Conclusion YDXNT may play an anti-neointimal hyper-plasia role by regulating AKT expression and phosphorylation.
yin dan xin nao tong soft capsuleneointimal hyperplasianetwork pharmacologymolecular doc-king
万方数据
维普
