Study on the effect of meihuashi complex bacteria product on renal stone for-mation and renal function in rats
Objective To investigate the effect of Meihuashi complex bacteria product(MCBP)on the inhibition of calcium oxalate stone formation and the protection of renal function in rats.Methods Twenty four SPF grade male rats(10 weeks old)were randomly divided into the control group(normal drinking water and feed,n=6),model group(1％ethylene glycol,n=6),potassium sodium hydrogen citrate(PSHC)treatment group(1％ethylene glycol+0.9 g/kg sodium potassium hydrogen citrate,n=6)and MCBP treatment group(1％ethylene glycol+0.4 g/kg enzyme fossil compound bacteria powder,n=6).On days 0 and 28,the concentration of u-rea,creatinine and Ca2+in serum as well as the concentration of oxalic acid and Ca2+in urine were measured.HE and immunohistochemical staining were used to detect factors(NF-κB P65,STAT3,TNF-α and IL-1β)in the renal tissues.Other indicators were also measured,including malondialdehyde(MDA),superoxide dis-mutase(SOD),glutathione peroxidase(GSH-Px)and catalase(CAT)in serum.Results The results of HE staining demonstrates that in the model group,lumina of the renal tubules was filled with calcium oxalate crys-tals,while the condition was greatly improved in the MCBP and PSHC treatment group.There was no significant difference in the concentration of urea,creatinine and Ca2+in serum as well as the concentration of oxalic acid and Ca2+in urine among all groups on D 0.However,on D 28,such indicators of the model group was greatly elevated in comparison with those of the control group(P＜0.05).Compared with the model group,the concen-tration of oxalic acid and Ca2+in urine and the concentration of urea and creatinine in serum in the MCBP and PSHC treatment group was all decreased with statistical difference(P＜0.05).Compared with the control group,CAT,GSH-Px and SOD levels in the model group were all decreased,while the MDA level was increased(P＜0.05).Compared with the model group,CAT,GSH-Px and SOD levels were increased in the MCBP and PSHC treatment group(P＜0.05),but the MDA levels was decreased.Compared with the control group,the expression of NF-κB p65,STAT3,TNF-α and IL-1 β in renal tissues was increased in the model group(P＜0.05).Compared with the model group,the expression of NF-κB p65,STAT3,TNF-α and IL-1β was de-creased in the MCBP and PSHC treatment group(P＜0.05).Conclusion Meihuashi complex bacteria product can reduce oxalic acid and Ca2+content in urine and enhance antioxidant activity.It has inhibitory effects on kid-ney stone formation and protective effects on renal function.
万方数据
维普
