Oral acute toxicity test of Aspongopus chinensis decoction
Objective To determine the acute toxicity of Aspongopus chinensis decoction(ACD)on mice,and to evaluate its safety,so as to provide evidence for safe drug use.Methods According to the Technical Guiding Principle of Acute Toxicity Study of Traditional Chinese Medicine and Natural Medicine([Z]GPT2-1),the a-cute toxicity of the decoction was observed by maximum tolerance dose method.Kunming mice were randomly di-vided into two groups:control group and drug administration group,with 20 mice in each group,half male and half female.For 14 days,the control group was given normal saline(NS)and the administration group was giv-en ACD(20 g/kg).The poisoning symptoms and reaction of mice was observed.The weight of mice and the feed and water consumption was recorded every two days.Then the mice were dissected,the organs were weighed and HE staining was performed,and serum was collected for the detection of ALT,AST,Cre,BUN in-dexes.Results Compared with the control group,the experimental group had no toxic symptoms and abnormal reaction,and there were no significant differences in body weight,food and water intake,organ coefficients,ALT,AST,Cre,and BUN.No pathological abnormalities were observed in liver,spleen,kidney,stomach,tes-tis,and ovary after administration.Conclusion The MTD(maximum tolerance dose)of ACD was＞20 g/kg,which had no lethal effect with high safety.
万方数据
维普
