首页|淫羊藿苷对锰暴露SD大鼠血清睾酮含量及睾丸间质细胞损伤修复的作用

淫羊藿苷对锰暴露SD大鼠血清睾酮含量及睾丸间质细胞损伤修复的作用

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
目的 探讨淫羊藿苷(ICA)对锰(Mn)暴露睾丸间质细胞损伤的修复作用及其对SD大鼠血清睾酮含量的影响。方法 SD 大鼠随机分为对照组、模型组(30mg/kg MnCl2·4H2O)、ICA 干预组(30 mg/kg MnCl2·4H2O 加 100 mg/kg ICA);连续4周(每周5 d)腹腔注射MnCl2·4H2O，随后ICA干预组连续2周灌胃ICA;采用HE染色法观察睾丸结构变化;Elisa法检测大鼠血清睾酮含量(TSTO)，qPCR法检测大鼠睾丸3β-HSD、StAR和P450scc的表达。小鼠间质细胞系TM3随机分为对照组、模型组(1 mmol/L Mn2+)、ICA 干预组 I(1 mmol/L Mn2++0。08 μmol/L ICA)、ICA 干预组 Ⅱ(1 mmol/L Mn2++0。4μmol/L ICA)、ICA 干预组Ⅲ(1 mmol/L Mn2++2 μmol/L ICA)、ICA 干预组Ⅳ(1 mmol/L Mn2++10 μmol/L ICA)、ICA 干预组V(1 mmol/L Mn2++50 μmol/L ICA);采用MTT法检测不同浓度ICA对锰暴露TM3细胞体外增殖的影响。结果 ICA可修复锰对生精小管结构的破坏;锰可降低大鼠血清睾酮浓度，ICA可提高锰暴露大鼠血清睾酮浓度;锰可抑制睾丸中睾酮生成相关基因3β-HSD、StAR和P450scc的表达，ICA可促进3β-HSD、StAR和P450scc的表达;锰可抑制TM3体外增殖，ICA可促进锰暴露TM3体外增殖。结论 锰可抑制StAR、P450scc和3β-HSD表达，抑制睾丸间质细胞合成睾酮;ICA可促进StAR、P450scc和3β-HSD表达，促进锰暴露睾丸间质细胞合成睾酮;ICA可修复锰对睾丸间质细胞的损伤。
Effects of Icariin on serum testosterone levels and repair of leydig cell line damage in manganese-exposed SD rats
Objective To investigate the repairing effect of Icariin(IC A)on manganese(Mn)-exposed testicu-lar Leydig cell injury and its effect on serum testosterone content in SD rats.Methods SD rats were randomly di-vided into a control group,a model group(30 mg/kg MnCl2·4H2O),and an IC A intervention group(30 mg/kg MnCl2·4H2O+100 mg/kg ICA);MnCl2·4H2O was injected intraperitoneally for 4 consecutive weeks(5 days per week),followed by ICA gavage for 2 consecutive weeks in the ICA intervention group;HE staining was used to observe testicular structural changes;Elisa assay for serum testosterone(TSTO)and qPCR assay for the expression levels of 3β-HSD,StAR and P450scc in rat testis.Mouse Leydig cell line TM3 was randomly divided into control group,model group(1 mmol/L Mn2+),ICA intervention group I(1 mmol/L Mn2++0.08μmol/L ICA),IC A intervention group Ⅱ(1 mmol/L Mn2++0.4μmol/L ICA),ICA intervention group Ⅲ(1mmol/L Mn2++2μmol/L ICA),ICA intervention group Ⅳ(1 mmol/L Mn2++10μmol/L ICA),ICA inter-vention group V(1 mmol/L Mn2++50μmol/L ICA);the effects of different concentrations of ICA on the pro-liferation of manganese exposed TM3 cells in vitro were detected by MTT method.Results ICA can repair the damage of manganese to the structure of spermatogenic tubules;manganese can reduce the serum testosterone concentration in rats,and ICA can increase the serum testosterone concentration in manganese-exposed rats;manganese can inhibit the expression of testosterone production-related genes 3β-HSD,StAR,and P450scc in the testes,and ICA can promote the expression of testosterone production related genes 3β-HSD,StAR,and P450scc in the testes of manganese-exposed rats;manganese can inhibit TM3 proliferation in vitro,and ICA can promote TM3 proliferation in vitro in manganese exposed rats.ICA promoted the expression of 3β-HSD,StAR and P450scc in the testis of manganese-exposed rats;manganese inhibited the proliferation of TM3 in vitro,and ICA promoted the proliferation of TM3 in vitro.Conclusion Manganese can inhibit the expression of StAR,P450scc and 3β-HSD,and inhibit the synthesis of testosterone in testicular stromal cells.ICA can promote the expression of StAR,P450scc and 3β-HSD,and promote the synthesis of testosterone in manganese-exposed testicular stromal cells.ICA can repair the damage of manganese on testicular interstitial cells.

manganeseIcariinleydig cellstestosterone

于光远、王俊淑、项雯鑫、钟红群、田莹、檀军、张继东

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遵义医科大学组织胚胎学教研室,贵州遵义 563099

遵义医科大学医学与科技学院基础医学院,贵州遵义 563099

遵义医科大学形态学实验室,贵州遵义 563099

遵义医科大学免疫学教研室,贵州遵义 563099

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锰 淫羊藿苷 睾丸间质细胞 睾酮

国家自然科学基金遵义市科技与大数据局遵义医科大学联合资金项目贵州省科技厅项目

31960156遵市科合HZ字2023189号黔科平台人才20211350-025

2024

遵义医科大学学报
遵义医科大学

遵义医科大学学报

CSTPCD
ISSN：2096-8159
年,卷(期)：2024.47(4)
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