Effect of kinase ULK1 on apoptosis of acute T lymphocytic leukemia cells
Objective To investigate the effect of ULK1 on apoptosis of acute T lymphocytic leukemia cell line CEM-C7 and its relationship with AMPKα/ULK1 axis.Methods The CEM-C7 cells were treated with ULK1 ac-tivator LYN 1604(0,1.2,and 2 μmol/L),and ULK1 inhibitor MRT68921(0,1.2,and 2 μmol/L)for 24 h.The apoptosis rate of the CEM-C7 cells was detected by flow cytometry.The drug concentration with the most obvious apoptosis effect was screened for follow-up experiments.Edu and Soft agar tests were conducted to detect its effect on the proliferation of CEM-C7 cells,flow Bax cytometry was detected to explain its effect on the mito-chondrial membrane potential of CEM-C7 cells,and the changes on the nucleus and mitochondrial ultrastructure of the CEM-C7 cells were observed under transmission electron microscopy.The expressions of ULK1,p-ULK1(Ser317),Caspase3,Cleaved caspase3,Bcl-2,Bax,PCNA,AMPKα,and p-AMPKα(Ser172)were detected by Western blot analysis after activation and inhibition of ULK1.Results After 24 h intervention with ULK1 in-hibitor and activator,compared with the control group,ULK1 inhibitor could significantly promote the apoptosis of CEM-C7 cells in a dose-dependent manner,inhibit the proliferation of CEM-C7 cells,and decrease the mem-brane potential of mitochondria,with statistical significance(P＜0.05).The apoptosis could be observed by transmission electron microscope.The protein levels of p-ULK1(Ser317)and p-AMPKα(Ser172)were de-creased,but ULK1 activator had no significant effect on the CEM-C7 cells.Conclusion ULK1 may affect the ap-optosis of CEM-C7 cells through AMPKα/ULK1 axis.
Unc51-like autophagy activating kinase 1acute T lymphocytic leukemiaCEM-C7 cellsapopto-sis
国家科技期刊平台
NSTL
万方数据
维普
