Establishment of real time fluorescence quantitative PCR assay for mpox virus
Objective To establish a real-time fluorescent quantitative polymerase chain reaction(RT-PCR)de-tection method for mpox virus.Methods Using the F3L gene of mpox virus as the target sequence,specific prim-ers and probes were designed to establish a mpox virus RT-PCR detection method,and the sensitivity,specificity and repeatability of the method were tested;Clinical samples of suspected mpox positive human pus swabs were detected and the clinical samples detection ability of the RT-PCR method were evaluated.Results The RT-PCR detection method established in this study can achieve specific detection of mpox virus,which has no cross reac-tivity with other Orthopoxvirus such as smallpox,vaccinia,and cowpox.The minimum detection limit is 103 cop-ies/μL.It can accurately detect mpox virus in human skin swabs samples.Conclusion This study established an RT-PCR detection method for mpox virus,which can quickly,specifically,and sensitively detect mpox virus in clinical samples.
mpox virusreal time fluorescence quantitative PCRrapid detection methodF3L gene
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