Antitumor effect of DM-[Ch][Tau]in the treatment of transplanted H22 liver cancer in mice
Objective To investigate the inhibitory effect of 5,6-Dibromonorcantharidin Monoeste(DM)and ionic liquid choline-taurinate([Ch][Tau])on H22 hepatocellular carcinoma transplant tumor in mice.Methods CCK-8 was used to assess the impact of DM,[Ch][Tau],and their combination on H22 hepatocellu-lar carcinoma cells.Subcutaneous inoculation of H22 cells was used to establish a mouse model of transplantation tumors.The 25 BALB/c tumor-bearing mice was randomly divided into the model(Saline),[Ch][Tau],Nor-cantharidine,DM,and DM-[Ch][Tau]groups.The medication was given every other day for one week,and body weight and transplanted tumors were observed.The tumor volume,tumor weight,and tumor suppression rate were calculated.Tumor tissue was examined using HE staining,and fluorescent TUNEL staining was per-formed to detect apoptosis.Immunohistochemical staining was performed to determine the expression of Bax and Bcl-2 in tumors.Results DMand[Ch][Tau]showed inhibitory effects on the proliferation of H22 cells,with IC50 of 1.13 μg/mL and 234.30 μg/mL respectively,and DM-[Ch][Tau](300 μg/mL)showed stronger in-hibition than DM,with IC50 of 0.84 μg/mL.Compared with the model group,the tumor suppression rates in[Ch][Tau],Norcantharidine,DM,and DM-[Ch][Tau]groups were 6.81%,27.82%,39.07%,64.55%,respectively.Tumors of[Ch][Tau]group grew rapidly after the administration of the drug.Tumors in Norcan-tharidine and DMgroups grew slowly with some inhibition.Tumor growth in the DM-[Ch][Tau]group was sig-nificantly inhibited,with extensive necrosis of tumors,and tumor growth was significantly lower.Spotty necrosis of tumors was visible in all groups,and the necrotic areas were DM-[Ch][Tau]＞DM＞Norcantharidine＞[Ch][Tau]＞model.Apoptosis rates were(17.66±13.31)%,(1.05±1.32)%,(26.31±11.89)%,(46.97±19.18)%,and(52.85±24.737)%in[Ch][Tau],model,Norcantharidine,DM,and DM-[Ch][Tau]groups,respectively.All groups induced apoptotic gene Bax to various extents,and the ratio of Bax/Bcl-2 was the highest in DM-[Ch][Tau]group,with the strongest apoptosis effect on tumor cells.Conclusion DM-[Ch][Tau]injection demonstrated anti-tumor effects in H22 hepatocellular carcinoma-bearing mice,providing evidence for the development of DM-[Ch][Tau]injection in chemotherapy.
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