Research on in vitro models of cachexia in myoblast cells caused by colorectal cancer
Objective Cachexia,as a systemic wasting disease,is characterized by an irreversible loss of skele-tal muscle mass,which seriously impairs the health of patients.Further understanding the mechanism of skeletal muscle wasting caused by cachexia and exploring potential therapeutic targets are important strategies to improve the quality of life of cachexia patients.Methods Incucyte was used to continuously observe the effects of tumor cell supernatant on the proliferation and migration of myoblasts and flow cytometry was used to detect the changes of apoptosis of myoblasts.RT-qPCR was used to detect the expression of myogenic differentiation,muscle atro-phy,and lipid peroxidation-related genes in the tumor cell supernatant.Cell imaging and flow cytometry were used to detect mitochondrial damage in myoblasts induced by tumor cell supernatant.Results By simulating the effect of cachexia on myoblasts and myotubes in vitro,it was observed that the migration ability of myoblasts was weakened and apoptosis was aggravated under the stimulation of tumor supernatant.After induction,the expres-sion of myogenic genes was down-regulated,and the expression of muscle atrophy-related genes was up-regula-ted,accompanied by lipid peroxidation.Further studies showed that the mitochondrial membrane potential of myoblasts changed significantly.Conclusion Cachexia can cause skeletal muscle atrophy by inducing lipid peroxi-dation and mitochondrial damage.Protecting mitochondria from cachexia factors may be a potential strategy for the treatment of skeletal muscle loss in cachexia.
万方数据
维普
