Improvement and mechanism of gastrodin on learning and memory in acute neuroinflammation rats
Objective To observe the improvement effect of Gastrodin(GAS)on the neurocognitive function in rats with acute neuroinflammation and explore its mechanism.Methods Fifty adult SD male rats were randomly divided into three groups,blank group(Con group,n=10),LPS group(L group,n=20),and LPS+GAS group(L+G group,n=20).The rats of the Con group were injected intraperitoneally with saline;the L group was injected intraperitoneally with lipopolysaccharide(LPS)at a dose of 5 mg/kg to establish an acute neuroin-flammation model;the L+G group was given GAS at a dose of 30 mg/kg by gavage after intraperitoneal injection of LPS.The L and L+G groups were further divided into two subgroups of 7 d and 14 d according to the dura-tion of LPS action and frequency of GAS gavage(n=10).The following experiments were performed respective-ly:(1)Place navigation test(PNT)with 5 d Morris water maze(MWM)before model construction,and spatial probe test(SPT)with MWM after modeling;(2)Detect the levels of IL-1 β and TNF-α in rat hippocampal tis-sue by ELISA.(3)Immunohistochemistry was used to detect the expression of Tau,P-Tau(Ser202),and Aβproteins in rat hippocampus.Results(1)Behavioral tests:Before modeling,the escape latency of rats in each group was gradually shortened(P＜0.05).After the model was successfully constructed,the number of plat-form traversals,the number of effective area traversals,the percentage of target quadrant traversals,and the per-centage of retention time of the rats in the L7 d an d L14 d groups were significantly reduced(P＜0.05),and the percentage of target quadrant traversals in the(L+G)7 d group was reduced(P＜0.05);after GAS ga-vage,the number of platform traversals,the number of effective area traversals,and the percentage of retention time of the rats in the(L+G)14 d group were increased(P＜0.05)compared with the L14 d group.(2)Com-pared with the Con group,the hippocampal tissue expression of inflammatory factors TNF-α and IL-1 β was ele-vated in the L7d,(L+G)7 d,and L14 d groups of rats(P＜0.05),and the hippocampal tissue expression of inflammatory factor IL-1 β was elevated in the(L+G)14 d group(P＜0.05).Compared with the L14 d group,TNF-α and IL-1 β expression was reduced in the(L+G)14 d group(P＜0.05).(3)Compared with the Con group,Tau and Aβ protein expression was increased in the hippocampus of the L7 d group(P＜0.05),and Tau,P-Tau,and Aβ protein expression was increased in the L14 d group(P＜0.05).After GAS gavage,the expression of P-Tau,Tau,and Aβ proteins in the(L+G)14 d group was decreased compared with the L14 d group(P＜0.05).Conclusion GAS can improve neurocognitive function in rats with acute neuroinflammation,and the mechanism may be related to anti-inflammation,inhibition of Tau protein phosphorylation,and reduction of Aβ protein deposition.
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