Long-term hyperoxia-induced intestinal dysbiosis in mice was studied based on 16s rRNA sequencing
Objective A mouse model of long-term hyperoxia was established by oxygen inhalation,and the effects of long-term hyperoxia on intestinal microbiota were explored by 16s rRNA sequencing;antibiotic treated (ABX)model was also performed to investigate how gut microbiome modulate hyperoxia-induced gut injury.Methods 16 C57BL/6 male mice were randomly divided into hyperoxia and control groups exposed to 80％ oxy-gen and room air for 14 days,respectively.Fecal pellets were collected for 16s RNA sequencing.In addition,ABX and SPF mice were exposed to hyperoxia and room air,respectively,and intestinal gut injury was analyzed.Results Hyperoxia significantly increased the cyanobacteria and actinobacteria,and altered the β diversity of in-testinal flora.Erysipelotrichaceae and actinobacteria in the hyperoxia group were the marker bacteria,while muri-baculaceae in the control group were the marker bacteria.ABX significantly reduced the hyperoxia-induced gut injury and immune response (LPS/TLR-4).Conclusion Long-term hyperoxia induces gut dysbiosis in mice,in-creasing relative abundance of aerobes and decreasing obligate anaerobes.Gut microbiome may contribute to hy-peroxia-induced gut injury through LPS/TLR-4 pathway.
万方数据
维普
