Objective To explore the role of central amygdala(CeA)in isoflurane anesthesia-induced changes of consciousness and the mechanism involved.Methods In this study,12 healthy male C57BL/6N mice were in-cluded.The experimental group was injected with ibotenic acid damaged virus (IBO,n=6 ),and the control group was injected with normal saline (NS,n=6).EEG electrodes were used to establish the damaged experi-mental model.After 7 days,the lost of righting reflex (LORR),recovery of the righting reflex (RORR),and cortical EEG changes were recorded.Results Neuronal activity in the CeA was activated during isoflurane anes-thesia.Compared with the NS group,IBO disruption of the CeA prolonged the isoflurane anesthesia (t=-2.898,P<0.05 ),the EEG showed decreased δ waves (t=6.037,P<0.05 ),increased β waves (t=-2.257,P<0.05),isoflurane anesthesia recovery time was shortened (t=2.230,P<0.05),and EEG showed increased γ waves (t=-2.495,P<0.05).Conclusion Disruption of CeA can inhibit the anesthesia effect of isoflurane and regulate the process of changing consciousness caused by anesthesia.
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