Astragalus flavones improved the inflammatory response of mice with acute myocardial infarction by down-regulating NF-κB and p38 MAPK signaling pathways
Objective To investigate the improvement of myocardial inflammation in mice with acute myocardial infarction by down-regulation of NF-κB and p38 MAPK signaling pathways by total flavonoids of astragalus (TFA)in Mongolia.Methods Forty C57BL/6J mice were randomized into Sham group,model group,TFA group,and metoprolol-positive drug group by random number column method,and the left anterior descending branch of coronary artery was ligated to establish an acute myocardial infarction model,and the mice were given the drug by continuous gavage for 10 days.Then electrocardiograms of the mice,biochemistry of the serum,and HE staining were carried out;and the protein expression of NF-κB p65,p38 MAPK/p-p38 MAPK,TNF-α,and JAK1/STAT1 in the myocardial tissues of mice was detected by western blotting.Results After acute myocardial infarction mice were treated with TFA,the fluctuation of PR interval of mouse ECG was normal,the ST segment of QT interval was slightly depressed,the T wave was not inverted,the expression level of serum creatine kinase MB isoenzyme (CK-MB)and myoglobin (MYO/MB)was down-regulated,and the degree of damage to myocar-dial tissues was significantly reduced,with a decrease in cell rupture,and only a localized and scattered infiltra-tion of inflammatory cells.The results of western blotting showed that the protein expression of JAK1,STAT1,NF-κB p65,TNF-α,and p38 MAPK/p-p38 MAPK tended to decrease significantly after treatment by TFA drugs.Conclusion TFA attenuates acute myocardial infarction injury in mice,which may act by inhibiting the NF-κB and p38 MAPK signaling pathways,reducing myocardial inflammation and thus ameliorating myocardial infarction.
myocardial infarctiontotal flavonoids of astragalusinflammationnuclear transcription factor κBmitogen-activated protein kinase
万方数据
维普
