Preparation and optimization of aloe emodin and ligustrazine nanoliposomes
Objective:The objective of this study was to prepare liposome nanoparticles containing aloe emodin and ligustrazine(AE-TMP-LIP),optimize the formulation,and determine the optimal processing conditions for AE-TMP-LIP.Methods:Liposomes were prepared using the film dispersion method.The UV absorbance of AE and TMP was determined using UV spectrophotometry.The prescription and preparation process were optimized using a star design-effect surface method,with investigation factors including solvent selection,solvent dosage,temperature,and other single factors.The drug content in the liposomes was used as an index to verify the results.Results:Among all investigated factors,changes in temperature at 45℃,amount of ultra-pure water used,and amount of film forming solvent had the greatest influence on liposome content.Optimal process optimization conditions were determined through a star design-effect surface method involving three factors at three levels.Conclusion:To prepare AE-TMP-LIP,it is recommended to use 1 mg of aloe emodin and ligustrazine each,along with 30 mg soy lecithin,10 mg cholesterol,13 mg Tween-80.These ingredients were dissolved in trichloromethane methanol organic solvent(4∶1 ratio),followed by ultrasonication.After complete film formation under rotary pressure evaporation at a temperature of 45℃,adjust hydration temperature to 35℃.Once lipid films are completely dissolved and cooled to room temperature,add 5 mL ultra-pure water for atmospheric hydration.The resulting liposomes should be light yellow in color,clear and transparent,with an average particle size of(146±18)nm,and dispersion index(PDI)of 16.8±3.2.
Aloe emodintetramethylpyrazinenanoliposomesolubilityoptimization of formulation
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