芒柄花黄素对非小细胞肺癌A549荷瘤裸鼠肿瘤组织细胞凋亡的影响及其机制
Effect of formononetin on apoptosis of tumor cells in non-small cell lung cancer A549 nude mice and its mechanism
张丽君 1欧逢莲 2张焱 2黄国玉 2肖纪余 2刘青阳 2许光亚2
作者信息
- 1. 大理大学药学院,云南 大理 671003;成都大学基础医学院,四川 成都 610083
- 2. 成都大学基础医学院,四川 成都 610083
- 折叠
摘要
目的:研究芒柄花黄素对人非小细胞肺癌 A549 所致荷瘤裸鼠肿瘤组织细胞凋亡及相关 Bax、Bcl-2、Caspase-3蛋白表达的影响.方法:将采用皮下接种 A549 细胞建立的 A549 荷瘤小鼠随机分为模型组、阳性对照组(环磷酰胺 20 mg·kg-1)及芒柄花黄素高、中、低剂量组(80、40、20 mg·kg-1),每天灌胃给药,持续 3 周.测定各组裸鼠肿瘤组织大小并计算其体积,Tunel 荧光染色和流式细胞术分析肿瘤组织细胞凋亡情况,RT-qPCR 和 Western blot 检测凋亡相关因子 Bax、Bcl-2、Caspase-3蛋白及 mRNA表达水平.结果:与模型组比较,各浓度芒柄花黄素呈剂量依赖性降低裸鼠肿瘤体积(P<0.05);促进了肿瘤组织细胞的凋亡(P<0.05).芒柄花黄素高剂量组(80 mg·kg-1)显著增加组织中 Bax、Caspase-3蛋白及 mRNA表达,降低 Bcl-2 蛋白及 mRNA表达(P<0.05).结论:芒柄花黄素能显著抑制 A549 荷瘤裸鼠肿瘤组织的生长,其作用机制与降低抗凋亡蛋白 Bcl-2的表达、促进促凋亡蛋白 Bax和Caspase-3的表达有关.
Abstract
Objective:To investigate the effect of formononetin on cell apoptosis and expressions of Bax,Bcl-2 and Caspase-3 in tumor tissues of nude mice induced by human non-small cell lung cancer A549.Methods:A549 tumor-bearing mice established by subcutaneous inoculation of A549 cells were randomly divided into model group,positive control group(cyclophosphamide,25 mg·kg-1),and formononetin high,medium and low dose groups(80 mg·kg-1,40 mg·kg-1,20 mg·kg-1).The mice were administrated by gavage every day for 3 weeks.The tumor tissue size was measured and its volume was calculated.Tunel staining was used to observe the apoptosis of tumor tissue.Flow cytometry was used to analyze the apoptosis rate of tumor tissue related cells,and RT-qPCR and Western blot were used to detect the protein and mRNA expression levels of apoptosis-related factors Bax,Bcl-2 and Caspase-3.Results:Compared with the model group,the tumor volume of nude mice was decreased in a dose-dependent manner by each concentration of formononetin(P<0.05).Formononetin promoted the apoptosis of tumor cells(P<0.05).The expressions of Bax and Caspase-3 protein and mRNA were significantly increased in high-dose group(80 mg·kg-1),whereas the expressions of Bcl-2 protein and mRNA were decreased(P<0.05).Conclusion:Formononetin can significantly inhibit the growth of tumor tissue in A549 tumor-bearing nude mice.The mechanism of action is related to reducing the expression of anti-apoptotic protein Bcl-2 and promoting the expression of pro-apoptotic proteins Bax and Caspase-3.
关键词
芒柄花黄素/人非小细胞肺癌/Bax蛋白/Bcl-2蛋白/Caspase-3蛋白Key words
Formononetin/human non-small cell lung cancer/Bax Protein/Bcl-2 protein/Caspase-3 protein引用本文复制引用
出版年
2025
国家科技期刊平台
NSTL
万方数据