目的:研究黄芩苷-栀子苷(BG)对脑缺血再灌注后继发肺损伤的干预作用,并探讨其对肺部 HMGB1/PI3K/AKT 蛋白表达的影响.方法:大鼠分为空白组、脑缺血再灌注模型组、BG 低剂量组和 BG 高剂量组.除空白组外,其余组建立脑缺血再灌注模型.记录体质量,观察肺部情况,检测肺泡灌洗液总蛋白、MDA 和 SOD 水平,使用免疫荧光和 Western Blot 法检测 HMGB1 和相关蛋白表达.结果:与空白组比较,模型组大鼠体质量下降,肺组织受损,肺泡灌洗液中总蛋白含量上升,肺组织 MDA 水平上升、SOD 水平下降.而 BG 高低剂量组(50、25 mg·mL-1)能缓解这些变化,且呈现剂量依赖性.免疫荧光和 Western Blot 结果显示,模型组 HMGB1 及相关蛋白表达增加,BG 给药组表达降低.结论:BG 对脑缺血再灌注后继发的肺损伤具有干预作用,可能通过调节抗氧化酶活性、抑制 HMGB1/PI3K/AKT通路相关蛋白表达,减轻大鼠脑缺血再灌注后继发肺损伤.
Baicalin-Geniposide modulates the HMGB1/PI3K/AKT signaling pathway to reduce lung injury following cerebral ischemia-reperfusion
Objective:To investigate the intervention effect of Baicalin-Gardenoside(BG)on secondary lung injury following cerebral ischemia-reperfusion and explore its influence on the expression of HMGB1/PI3K/AKT proteins in the lungs.Methods:Rats were divided into blank group,cerebral ischemia-reperfusion model group,low-dose BG group,and high-dose BG group.Except the blank group,the remaining groups all underwent cerebral ischemia-reperfusion modeling.Body weight was recorded,and lung conditions were observed.Levels of total protein,MDA,and SOD in lung lavage fluid were measured.The expression of HMGB1 and related proteins was detected using immunofluorescence and Western Blot methods.Results:Compared with the blank group,rats in the model group showed decreased body weight,lung tissue damage,increased total protein in lung lavage fluid,elevated MDA levels,and decreased SOD levels in lung tissue.However,the BG high and low-dose groups(25 and 50 mg·mL-1)alleviated these changes,showing a dose-dependent relationship.Immunofluorescence and Western Blot results revealed increased expression of HMGB1 and related proteins in the model group,which was reduced in the BG treatment groups.Conclusion:BG exhibited an intervention effect on secondary lung injury following cerebral ischemia-reperfusion,possibly by modulating antioxidant enzyme activity,inhibiting the expression of HMGB1/PI3K/AKT pathway-related proteins,and alleviating secondary lung injury in rats after cerebral ischemia-reperfusion.
Baicalingeniposidesecondary lung injury after cerebral ischemia-reperfusionlung injuryHMGB1PI3K/AKT
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